Binding of fluorescence- and gold-labeled oligodeoxyribonucleotides to cytoplasmic intermediate filaments in epithelial and fibroblast cells

Experimental Cell Research
R HartigP Traub

Abstract

Previously, in vitro experiments have demonstrated the capacity of intermediate filaments (IFs) to associate with polyanionic compounds, including nucleic acids. To prove that this activity is also shown by IFs in quasi-intact cells, digitonin-permeabilized epithelial PtK2 and mouse fibroblast cells were treated with FITC-labeled, single-stranded oligodeoxyribonucleotides and analyzed, after indirect decoration of their IF systems with TRITC-conjugated antibodies, by fluorescence microscopy. While cytokeratin IFs exhibited a strong affinity for and exact codistribution with oligo(dG)25, vimentin IFs were less active in binding this oligonucleotide. Other oligonucleotides, like oligo(dT)25, oligo[d(GT)12G] and oligo[d(G3T2A)4G], were bound to IFs with lower efficiency. In general, the introduction of dA residues into oligo(dG)n or oligo(dGT)n tracts reduced the IF-binding potential of the nucleic acids. This, however, increased significantly upon reduction of the ionic strength to half physiological, indicating a strong electrostatic binding component. The binding reaction was often obscured by simultaneous association of the oligonucleotides with cellular membranes mostly in the perinuclear region, an activity that was largely ...Continue Reading

References

May 16, 1978·European Journal of Biochemistry·V G CorcesJ Avila
Sep 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·J B BryanK H Doenges
Jan 1, 1992·Progress in Nucleic Acid Research and Molecular Biology·J M Raats, H Bloemendal
Jul 3, 1992·Science·J D PuglisiJ R Williamson
Nov 1, 1990·The Journal of Cell Biology·J M RaatsH Bloemendal
Jan 1, 1990·Electron Microscopy Reviews·M Carmo-Fonseca, J F David-Ferreira
Sep 1, 1985·Cell·P M SteinertD R Roop
Jan 1, 1987·Cell Motility and the Cytoskeleton·K M Wolf, J M Mullins
Jan 1, 1989·Cell Motility and the Cytoskeleton·M W KlymkowskyA Domingo
Jan 1, 1988·Annual Review of Biochemistry·P M Steinert, D R Roop
Dec 1, 1987·Journal of Neuroscience Methods·S MittmanJ H Belgum
Jun 16, 1987·Biochemistry·J M Caron, R D Berlin
Mar 1, 1980·European Journal of Biochemistry·V G CorcesG Wiche
Oct 1, 1983·The Journal of Cell Biology·U AebiT T Sun
May 1, 1983·Hoppe-Seyler's Zeitschrift für physiologische Chemie·P Traub, W J Nelson
Jun 1, 1995·Current Opinion in Structural Biology·D S PilchK J Breslauer
Aug 1, 1995·The Journal of Cell Biology·K R OlsonJ B Olmsted
Mar 15, 1995·European Journal of Biochemistry·H Lütcke
Dec 7, 1994·Molecular and Cellular Biochemistry·D M BeltramoH S Barra
Jan 1, 1994·Annual Review of Biochemistry·E Fuchs, K Weber
Sep 1, 1994·Experimental Cell Research·J M Andreoli, K T Trevor
Feb 1, 1994·Current Opinion in Cell Biology·S Heins, U Aebi
Sep 1, 1993·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·R L Shoeman, P Traub
Feb 1, 1993·Current Opinion in Cell Biology·M Stewart

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Citations

May 23, 2002·DNA and Cell Biology·Guohong LiPeter Traub
Dec 13, 2007·Nucleic Acids Research·Alexander KirilyukGerald G Schumann
Feb 19, 2009·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Alessandro Di BellaEugenio Bertelli
Oct 9, 2003·The Journal of Urology·Simon A LewisChristian M Spilker
Jun 16, 2004·Journal of Controlled Release : Official Journal of the Controlled Release Society·Fuxin Shi, Dick Hoekstra
Mar 20, 1999·The Journal of Investigative Dermatology·A WaseemI M Leigh
Sep 24, 2004·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Alessandro CarapelliEugenio Bertelli
Jul 28, 2020·Investigative Ophthalmology & Visual Science·Gian Marco TosiEugenio Bertelli

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