PMID: 8593957Dec 1, 1995Paper

Binding of hemoglobin by Porphyromonas gingivalis

FEMS Microbiology Letters
A AmanoS Shizukuishi

Abstract

In this study, we investigated whether Porphyromonas gingivalis can bind hemoglobin as an initial step in the acquisition of heme from hemoglobin. The binding of human hemoglobin by P. gingivalis cells was determined using [3H]hemoglobin. Hemoglobin binding occurred rapidly, reversibly and specifically. A Scatchard analysis of the binding data generated a linear plot, indicating a single population of binding proteins. The apparent Kd was 1.0 +/- 0.19 x 10(-6) M and there were 3.2 +/- 0.76 x 10(4) binding sites per cell. Hemoglobin binding was inhibited by unlabeled human hemoglobin but not by hemin and protoporphyrin IX. The binding was only partially inhibited by human serum albumin, transferrin, lactoferrin, catalase and cytochrome c. These results suggest that the ligand recognized by the binding protein may not be the heme moiety. The binding of hemoglobin considerably increased when the organisms were grown under hemin-limited conditions. Hemoglobin bound to outer membrane proteins extracted from P. gingivalis cells on a dot blot binding assay and binding ability was lost after heating bacterial proteins. These results suggest that P. gingivalis cells interact with human hemoglobin through specific binding sites on their ...Continue Reading

References

Dec 1, 1992·Journal of General Microbiology·B C Lee, P Hill
Jan 1, 1991·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·S C Holt, T E Bramanti
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Citations

Dec 19, 2009·Periodontology 2000·Atsuo Amano
Jan 18, 2005·FEMS Microbiology Reviews·Teresa OlczakCaroline Attardo Genco
Aug 2, 2017·Periodontology 2000·Jeffrey L EbersoleOctavio A Gonzalez
Oct 8, 2003·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·Patrick S StaytonMichele Gilbert
May 29, 2002·Oral Microbiology and Immunology·S GuanS Shizukuishi
Mar 14, 2000·FEMS Microbiology Letters·S Fujimura, T Nakamura
Aug 26, 1998·Biochemical and Biophysical Research Communications·M KuboniwaS Shizukuishi

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