Binding of homogeneous cytochrome b5 to rat liver microsomes. Effect on N-demethylation reactions.

Biochimica Et Biophysica Acta
D L Cinti, J Ozols

Abstract

Incubation of rat homogeneous detergent-solubilized cytochrome b5 with rat liver microsomes resulted in specific binding of the hemoprotein which was rapidly reduced by NADH. The NADH cytochrome c reductase activity in these preparations increased in proportion to the amount of cytochrome bound. However, the extra-bound detergent-solubilized cytochrome b5 did inhibit NADPH-dependent N-demethylations, the NADH synergism and NADPH cytochrome P-450 reductase activity. Manganese protoporphyrin-apocytochrome complex when bound to microsomes in amounts equivalent to detergent-solubilised cytochrome b5 showed no effect on N-demethylation activity. Furthermore, the binding of cytochrome b5 preparations reconstituted from heme and apocytochrome b5 had no effect on either the NADPH-dependent N-demethylation of aminopyrene or ethylmorphine or the NADH synergism observed with rat liver microsomes. In addition, homogeneous cytochrome b5 eluted from three additional Sephadex G-100 columns showed no inhibitory effects when bound to liver microsomes. Spectral analyses of the acid-acetone extract of the hemoprotein showed an absorption peak at 278 nm suggesting that the homogeneous b5 contains contaminating amounts of tightly bound detergent wh...Continue Reading

References

Apr 1, 1972·Archives of Biochemistry and Biophysics·N Oshino, R Sato
Sep 23, 1974·Biochemical and Biophysical Research Communications·E G Hrycay, R W Estabrook
Mar 1, 1971·Archives of Biochemistry and Biophysics·A Hildebrandt, R W Estabrook
Jul 1, 1970·Biochemical Pharmacology·J B Schenkman, D L Cinti
Feb 16, 1972·Biochemical and Biophysical Research Communications·Y Ichikawa, J S Loehr
Jan 1, 1971·Journal of Biochemistry·N OshinoR Sato
Sep 15, 1971·FEBS Letters·S A. KamathK Ananth Narayan
Jun 1, 1963·Archives of Biochemistry and Biophysics·A NILSSON, B C JOHNSON

❮ Previous
Next ❯

Citations

Jun 21, 1991·Biochemical Pharmacology·T D Boyer, E Olsen
Sep 10, 2014·Biochemistry·Donghak KimF Peter Guengerich
Nov 15, 1992·Archives of Biochemistry and Biophysics·C J PattenC S Yang
Jun 1, 1985·Biochemical Pharmacology·E Canova-DavisL Waskell
Apr 11, 2009·Protein Expression and Purification·Vera KotrbováMarie Stiborová
Jan 1, 1988·Archives of Biochemistry and Biophysics·I GollyW Schartau
Sep 20, 1976·Biochemical and Biophysical Research Communications·D L CintiF Bronner
Jan 15, 1977·Archives of Biochemistry and Biophysics·I Jansson, J B Schenkman
Aug 2, 2005·Archives of Biochemistry and Biophysics·F Peter Guengerich
Sep 15, 2001·Archives of Biochemistry and Biophysics·A E AitkenB S Masters
Sep 1, 1976·Life Sciences·J B SchenkmanK M Robie-Suh

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.