Binding of influenza viruses to sialic acids: reassortant viruses with A/NWS/33 hemagglutinin bind to alpha2,8-linked sialic acid

Virology
Wenxin Wu, Gillian M Air

Abstract

We have examined the specificity of binding of A/NWS/33 hemagglutinin (HA), exploring the effects of fucosylation, changing the Gal-GlcNAc linkage between the second and third sugars, and binding affinity for alpha2,8-linked sialic acid. The HA of A/NWS/33(HA)-Tokyo/67(NA) (NWS-Tok, H1N2) virus binds to 3'-linked sialyllactose with 10-fold higher affinity than 3' sialyllactosamine and 3-fold higher affinity than 6' sialyllactosamine. The P227H mutation in A/NWS/33(P227H)(HA)-A/Memphis/31/98(NA) (NWS-Mem/98, H1N2) results in sevenfold lower affinity for 3' sialyllactose, but binding to 6' sialyllactosamine is unchanged. The apparent switch from 3' to 6' specificity is solely due to a loss of Siaalpha2,3 binding. Fucosylation of the third sugar and changing the linkage between second and third sugars had little effect on binding by NWS-Tok, but marked effects on A/NWS/33(P227H)(HA)-tern/Australia/G70c/75(NA) (NWS-G70c, H1N9) and NWS-Mem/98. NWS-Tok, NWS-G70c, and NWS-Mem/98 bind to alpha2,8-bisialic acid with high affinity. NWS-Mem/98 can also bind to alpha2,8-trisialic acid, but with lower affinity. Together, these data show that alpha2,8-linked sialic acid, fucosylation of the third sugar, and linkage between the second and thi...Continue Reading

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Citations

Mar 30, 2006·Antimicrobial Agents and Chemotherapy·Michael P MalakhovFang Fang
May 11, 2007·Virology Journal·Kshama KumariGillian M Air
Dec 15, 2010·Virology Journal·Brian KimbleDaniel R Perez
Feb 20, 2013·Applied Microbiology and Biotechnology·Bai-Xue LinYong Tao
May 5, 2011·International Journal of Pharmaceutics·Shailja TiwariSuresh P Vyas
Dec 18, 2009·Science in China. Series C, Life Sciences·Hong Zhang
Dec 20, 2015·The Journal of General Virology·Kathlyn LavalHans J Nauwynck
Sep 20, 2011·Biochimica Et Biophysica Acta·Teresa Janas, Tadeusz Janas
Oct 29, 2013·Scientific Reports·J C F M DortmansC A M de Haan
Jun 13, 2015·Journal of Virology·I N Ambepitiya WickramasingheM H Verheije
Apr 11, 2008·Virus Genes·Yan-Long CongJin-Hua Liu
Jun 16, 2005·The Journal of Biological Chemistry·James N ArnoldRobert B Sim
Jan 18, 2020·The Journal of Biological Chemistry·Chanakha K NavaratnarajahRoberto Cattaneo

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