Abstract
Membrane preparations from monkey and pig hypothalami bound [125I]insulin specifically. The binding appeared to be greater by preparations from anterior than posterior portions of the pig hypothalamus. Binding was time dependent, and its dissociation was first order with a half-time at 22 degrees C of 14 min. Desalanine insulin was as effective as native insulin in inhibiting the binding of [125I]insulin, while proinsulin was less effective and desoctapeptide insulin still less effective in accord with their biologic activities. Binding by membranes from cortex and thalamus appeared to be less than from hypothalamus. [125I]insulin was infused into an arterial split monkey brain preparation to determine if insulin that was blood borne bound specifically to the primate hypothalamus. Half the brain was perfused with [125I]insulin alone and the other half with [125I]insulin plus an excess of unlabeled insulin. Radioautography showed specific binding of insulin localized to the median eminence, infundibular nucleus, and microvessels. Thus, the monkey and pig hypothalami bind insulin with characteristics similar to those reported for known target tissues for insulin. Furthermore, insulin from the blood stream binds to specific anatom...Continue Reading
Citations
Sep 8, 2012·Molecular Neurobiology·Rasoul GhasemiAbolhassan Ahmadiani
Jan 1, 1986·Physiology & Behavior·C R Plata-SalamánN Shimizu
Apr 1, 1992·Physiology & Behavior·M K McGowanS P Grossman
May 1, 1985·Brain Research Bulletin·J A WeyhenmeyerA Killian
Feb 1, 1997·Theriogenology·J A Downing, R J Scaramuzzi
Jan 1, 1991·Neuroscience and Biobehavioral Reviews·C R Plata-Salamán
Feb 1, 1987·The Proceedings of the Nutrition Society·B I Posner
Apr 1, 1993·Zentralblatt für Veterinärmedizin. Reihe A·T RojkittikhunN Lundeheim
Nov 16, 2004·Journal of Neurosurgery·Franklin C Wagner
Oct 1, 1987·Journal of Neurochemistry·W KumJ D Young
Mar 1, 1988·Journal of Neuroscience Research·E Recio-Pinto, D N Ishii
May 1, 1984·Journal of Neurochemistry·N R ZahniserJ V Vinych
Jan 1, 1995·Clinical and Experimental Hypertension : CHE·M S MuntzelA L Mark
Oct 28, 2014·Frontiers in Endocrinology·Enrique BlázquezJuan Miguel Ruiz-Albusac
Jan 26, 2017·Neuroendocrinology·Weiyi ChenMichael A Cowley
Jul 1, 1986·Journal of Neurochemistry·G A SaviolakisG J Chader
Mar 2, 2002·Diabetes·Marianthe Hamilton-WesslerRichard N Bergman
Aug 11, 1987·Brain Research·B A MastersM K Raizada