Binding of IRBIT to the IP3 receptor: determinants and functional effects

Biochemical and Biophysical Research Communications
Benoit DevogelaereH De Smedt

Abstract

IRBIT has previously been shown to interact with the inositol 1,4,5-trisphosphate (IP3) receptor (IP3R) in an IP3-sensitive way. So far it remained to be elucidated whether this interaction was direct or indirect, and whether it was functionally relevant. We now show that IRBIT can directly interact with the IP3R, and that both the suppressor domain and the IP3-binding core of the IP3R are essential for a strong interaction. Moreover, we identified a PEST motif and a PDZ-ligand on IRBIT which were critical for the interaction with the IP3R. Furthermore, we identified Asp-73 as a critical residue for this interaction. Finally, we demonstrated that this interaction functionally affects the IP3R: IRBIT inhibits both IP3 binding and IP3-induced Ca2+ release.

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Citations

Dec 15, 2010·Neurochemical Research·Dongki YangShmuel Muallem
Jun 19, 2013·Biochemical and Biophysical Research Communications·Tien Manh TranKyungpyo Park
Feb 3, 2012·Physiological Reviews·Min Goo LeeShmuel Muallem
Apr 13, 2007·Physiological Reviews·J Kevin FoskettDon-On Daniel Mak
Nov 19, 2013·Pflügers Archiv : European journal of physiology·Jeong Hee HongShmuel Muallem
Feb 13, 2014·Biochimica Et Biophysica Acta·Hideaki AndoKatsuhiko Mikoshiba
Jan 6, 2018·The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology·Kyu Pil LeeDongki Yang
Jun 7, 2008·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Benoit DevogelaereHumbert De Smedt
Mar 30, 2017·Proceedings of the National Academy of Sciences of the United States of America·Katsuhiro KawaaiKatsuhiko Mikoshiba
Mar 3, 2007·The Journal of Biological Chemistry·Rachel AshworthSandip Patel
Mar 3, 2020·Biochimica Et Biophysica Acta. Biomembranes·Meng WangLi-Ming Chen
Jul 20, 2021·Physiological Reviews·Kellie A Woll, Filip Van Petegem
Nov 30, 2006·Science's STKE : Signal Transduction Knowledge Environment·Chi-Un Choe, Barbara E Ehrlich
Mar 5, 2014·Cold Spring Harbor Protocols·Tomas LuytenLudwig Missiaen

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