PMID: 7540197Jul 1, 1995Paper

Binding of lanthanides to cell membranes in the presence of ligands

Journal of Inorganic Biochemistry
D Bingham, M Dobrota

Abstract

The effect of a series of ligands on the binding of the lanthanide, europium (Eu), to rabbit intestinal cell membranes was investigated in vitro. When tested as Eu-ligand complexes (ratio of Eu:ligand, 1:2) of intermediate stability (log stability constant, log K1, for the reaction Eu + L = EuL, of about 7-12) such as Eu-citrate and Eu-nitrilotriacetate (NTA), Eu was available for uptake in a soluble form by intestinal brush-border membrane vesicles (BBMV) in phosphate- and bicarbonate-free solutions at pH 7.2. Ligands with lower log K1 did not maintain Eu in solution whilst those of higher affinity did not donate it to membranes. Generally, there was a clear relationship between log K1 of the Eu-ligand complex and the binding of Eu to BBMV. This relationship identifies ligands that can effectively donate Eu to vesicles under these conditions. BBMV uptake of Eu was due to binding at two sites. Binding to the diethylenetriaminepentaacetate (DTPA)-sensitive site predominated at 20 degrees C and uptake by the DTPA-insensitive site was enhanced at 37 degrees C. Only trace amounts of the bound Eu appeared to be internalized within the vesicles. In the presence of physiological concentrations of phosphate and bicarbonate in cell cult...Continue Reading

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Citations

May 27, 2006·Chemical Society Reviews·Simon P Fricker
Aug 13, 2014·Proceedings of the National Academy of Sciences of the United States of America·Lihong WangXiao Hua Huang
Jan 16, 2010·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Jin-Xia LiXiao-Gai Yang

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