Binding of mannose-binding protein to Klebsiella O3 lipopolysaccharide possessing the mannose homopolysaccharide as the O-specific polysaccharide and its relation to complement activation.

Infection and Immunity
G Z JiangTakashi Yokochi

Abstract

Lipopolysaccharide from Klebsiella pneumoniae O3, which possesses the mannose homopolysaccharide as the O-specific polysaccharide, exhibits an extraordinarily high ability to activate the human complement system. We isolated the mannose-binding protein with a Klebsiella O3 lipopolysaccharide affinity column. The protein isolated had a molecular mass of much higher than 200 kDa, and it consisted of subunits with an apparent molecular mass of 32 kDa. The NH2-terminal sequence of the 32-kDa subunits was completely consistent with a part of the amino acid sequence of human serum mannose-binding protein. In immunoblotting, an anti-mannose-binding protein monoclonal antibody was definitely reactive with the isolated protein with the higher molecular mass. The protein isolated was bound exclusively to lipopolysaccharides possessing the mannose homopolysaccharide, not to lipopolysaccharide possessing the heteropolysaccharides. Klebsiella O3 lipopolysaccharide did not exhibit a high anticomplement activity in the serum from which the mannose-binding protein was depleted. It was concluded that the serum factor that bound to Klebsiella O3 lipopolysaccharide may be mannose-binding protein and that it may play a crucial role in the strong c...Continue Reading

References

Mar 16, 1992·Biochemical and Biophysical Research Communications·M MatsushitaT Fujita
May 1, 1989·The Journal of Experimental Medicine·M KuhlmanR A Ezekowitz
Sep 15, 1989·The Biochemical Journal·M E TaylorJ A Summerfield
Jan 1, 1989·The Journal of Experimental Medicine·R A EzekowitzR A Byrn
Jan 1, 1973·Acta Chemica Scandinavica·M CurvallW Nimmich
Sep 1, 1983·Journal of Biochemistry·N KawasakiI Yamashina

❮ Previous
Next ❯

Citations

Oct 4, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Kei OnoYoshio Kuroki
Jun 17, 2016·Microbiology and Molecular Biology Reviews : MMBR·Michelle K Paczosa, Joan Mecsas
Feb 17, 2001·Infection and Immunity·H ChibaY Kuroki
Aug 18, 2009·International Immunology·Da-Ming ZuoZheng-Liang Chen
Jun 21, 2006·Microbiology and Immunology·Gantsetseg TumurkhuuTakashi Yokochi
Sep 6, 2011·Glycobiology·Anna SwierzkoCzeslaw Lugowski
Apr 9, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Sunita GulatiSanjay Ram
Feb 1, 1996·Current Opinion in Immunology·J EpsteinR A Ezekowitz
Sep 4, 1999·Molecular Immunology·Y TakayamaN Matsu-ura
Jun 1, 1997·Microbiology and Molecular Biology Reviews : MMBR·A Vázquez-Torres, E Balish

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.