Binding of MCF-7 cell mitochondrial proteins and recombinant human estrogen receptors alpha and beta to human mitochondrial DNA estrogen response elements

Journal of Cellular Biochemistry
Jin Q ChenJ D Yager

Abstract

Our previous studies have shown that 17beta estradiol (E2) enhances the transcript levels of mitochondrial DNA (mtDNA)-encoded genes and mitochondrial respiratory chain (MRC) activity via estrogen receptors (ER). Others have reported the presence of putative estrogen responsive elements (ERE) in human mtDNA (mtEREs) and detection of ERs in mitochondria of rat uterine and ovary cells. Recently, we demonstrated the E2-enhanced mitochondrial localization of ERalpha and ERbeta, and E2-induced mtDNA transcript levels in MCF-7 cells. In this study, we applied electrophoresis mobility shift assays (EMSAs) and surface plasmon resonance (SPR) to determine if mitochondrial extracts, recombinant human ERalpha (rhERalpha), and rhERbeta interact with mtEREs. Using EMSAs, we observed that ER-containing mitochondrial extracts bound to mtEREs and the binding was enhanced by E2, whereas the binding of mitochondrial proteins from ERbeta-deficient cells was almost undetectable. Both rhERalpha and rhERbeta bound to the mtEREs and their binding was altered by their respective antibodies. However, the ERalpha antibodies did not alter the binding of MCF-7 cell mitochondrial extracts to mtEREs whereas the binding MCF-7 and MDA-MB-231 cell mitochondria...Continue Reading

References

Jan 1, 1986·Journal of Steroid Biochemistry·S GreenP Chambon
Sep 1, 1965·Archives of Biochemistry and Biophysics·W D Noteboom, J Gorski
Sep 30, 1995·Annals of the New York Academy of Sciences·J FishmanN T Telang
Jun 11, 1996·Proceedings of the National Academy of Sciences of the United States of America·G G KuiperJ A Gustafsson
Jan 1, 1996·Annual Review of Pharmacology and Toxicology·J D Yager, J G Liehr
Apr 1, 1996·Steroids·C V DemonacosC E Sekeris
Nov 1, 1996·Carcinogenesis·H S Feigelson, B E Henderson
Apr 25, 1997·The Journal of Biological Chemistry·B J CheskisC R Lyttle
Sep 1, 1993·The Journal of Steroid Biochemistry and Molecular Biology·C DemonacosC E Sekeris
May 4, 1999·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·A G RatoS Ramos
Oct 30, 1999·Toxicological Sciences : an Official Journal of the Society of Toxicology·J ChenJ D Yager
Mar 24, 2000·Endocrine-related Cancer·R J SantenL Berstein
Apr 25, 2000·American Journal of Physiology. Heart and Circulatory Physiology·P ZhaiD R Gross
May 10, 2000·Nature Medicine·G Kroemer, J C Reed
Jul 11, 2000·European Journal of Cell Biology·K SchellerU Scheer
Aug 30, 2000·Journal of the National Cancer Institute. Monographs·J D Yager
Sep 8, 2000·Molecular Endocrinology·M RazandiE R Levin
Nov 22, 2000·American Journal of Physiology. Heart and Circulatory Physiology·P ZhaiD R Gross
Feb 13, 2001·The Journal of Steroid Biochemistry and Molecular Biology·J A Gustafsson, M Warner
Feb 15, 2001·Human Pathology·S MannM Younes
May 22, 2001·Archives of Biochemistry and Biophysics·P MoutsatsouC E Sekeris
Aug 14, 2001·Journal of Molecular Recognition : JMR·R L Rich, D G Myszka
Aug 22, 2001·American Journal of Physiology. Heart and Circulatory Physiology·P ZhaiD R Gross
Mar 19, 2002·The Journal of Steroid Biochemistry and Molecular Biology·Jose RussoIrma H Russo
Apr 23, 2002·Protein Science : a Publication of the Protein Society·Yasmina S N DayDavid G Myszka
Mar 26, 2003·The Journal of Steroid Biochemistry and Molecular Biology·Shiuan ChenChun Yang
Jul 15, 2003·Toxicological Sciences : an Official Journal of the Society of Toxicology·Jinqiang ChenJames D Yager

❮ Previous
Next ❯

Citations

Mar 12, 2009·Experimental Brain Research·Todd M MoweryPreston E Garraghty
Apr 28, 2005·Free Radical Biology & Medicine·Scott W Ballinger
Sep 3, 2013·Frontiers in Neuroendocrinology·Jamaica R RettbergRoberta Diaz Brinton
Jan 20, 2006·The New England Journal of Medicine·James D Yager, Nancy E Davidson
Dec 29, 2012·The Journal of Biological Chemistry·Prasad TammineniNaresh Babu Venkata Sepuri
Sep 8, 2007·Antioxidants & Redox Signaling·Walter MalorniFlavia Franconi
Oct 15, 2010·Brain : a Journal of Neurology·Carla GiordanoValerio Carelli
Jun 30, 2007·Clinical and Experimental Pharmacology & Physiology·Sue P Duckles, Diana N Krause
Jul 28, 2010·Molecular Endocrinology·Guangfeng ZhangHarish Srinivas
Oct 22, 2008·World Journal of Gastroenterology : WJG·Mamta KalraRashmi Kaul
Sep 24, 2014·Genome Biology and Evolution·Amit BlumbergDan Mishmar
Jun 18, 2015·Expert Opinion on Therapeutic Targets·Maximilian WenigerIrshad H Chaudry
Jul 10, 2010·Journal of Biomedicine & Biotechnology·E Kassi, P Moutsatsou
Mar 28, 2013·International Journal of Endocrinology·Monika Puzianowska-KuznickaJacek Polosak
Apr 22, 2011·Gynecological Endocrinology : the Official Journal of the International Society of Gynecological Endocrinology·Noor Ahmad ShaikQurratulain Hasan
Feb 6, 2014·Longevity & Healthspan·Michael C Velarde
Apr 18, 2015·Journal of Diabetes Research·Anisha A GupteDale J Hamilton
Jul 28, 2016·Biological Reviews of the Cambridge Philosophical Society·Rebecca E KochGeoffrey E Hill
Nov 6, 2009·Therapeutic Advances in Neurological Disorders·James W SimpkinsMeharvan Singh
Mar 15, 2012·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Jorge Sastre-SerraPilar Roca
Jan 10, 2018·Frontiers in Aging Neuroscience·Sandra ZárateRicardo Gredilla
Sep 14, 2018·Autophagy·Jin XiangMiao-Miao Qi
Sep 20, 2018·Annual Review of Genetics·R Stefan IsaacL Stirling Churchman
Nov 28, 2007·Journal of Neuroscience Research·Junghee LeeHoon Ryu
Nov 9, 2007·International Journal of Cancer. Journal International Du Cancer·Akihiro NaitoMasahiro Higuchi
Mar 18, 2008·IUBMB Life·Anna-Maria G Psarra, Constantine E Sekeris
Oct 11, 2008·Journal of Cellular Biochemistry·Carolyn M Klinge
Oct 28, 2005·Journal of Molecular Recognition : JMR·Rebecca L Rich, David G Myszka
Aug 8, 2006·ChemMedChem·Elizabeth HillardGérard Jaouen
Jul 5, 2005·Molecular Pharmacology·Chris StironeVincent Procaccio
Jul 30, 2005·Molecular Pharmacology·Thomas P Burris, Venkatesh Krishnan

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.