PMID: 2495336Apr 1, 1989Paper

Binding of metastatic colon carcinoma cells to liver macrophages

Journal of Leukocyte Biology
T GjøenS O Kolset

Abstract

The liver is frequently colonized by metastatic tumor cells despite its dense population of macrophages (Kupffer cells). We have studied the interactions between metastatic colon carcinoma cells (DHD) and syngeneic Kupffer cells under different experimental conditions in vitro. In an adhesion assay the binding of DHD cells to Kupffer cell monolayers was shown to be time and temperature dependent, reaching a maximum level after about 90 min of incubation at 37 degrees C. In contrast, only a low level of binding could be observed at 4 degrees C. The level of binding could be increased by pretreatment of the Kupffer cells with phorbol 12-myristate 13-acetate. A firm interaction between the two cell types was shown to be dependent on the presence of calcium- and trypsin-sensitive structures on the surface of the Kupffer cells. Pretreatment of the macrophages with the cytoskeletal inhibitors colchicine and cytochalasin B was also found to reduce significantly the binding of tumor cells. This binding was also inhibited to a large extent by D-mannose and N-acetyl-D-galactosamine. The Kupffer cells were not cytotoxic against the colon carcinoma cells.

Citations

Mar 1, 1990·The New England Journal of Medicine·B R Zetter
May 10, 2013·International Journal of Colorectal Disease·J KruseL I Partecke
Jan 1, 1993·Molecular Carcinogenesis·S M SchollJ W Pollard
Jul 1, 1992·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·C A Toth, P Thomas

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