Binding of microtubule-associated proteins (MAPs) to rat brain mitochondria: a comparative study of the binding of MAP2, its microtubule-binding and projection domains, and tau proteins

Cell Motility and the Cytoskeleton
V JancsikA Rendon

Abstract

Two major brain microtubule-associated proteins (MAPs), MAP2 and tau, were found to be able to bind to purified rat brain mitochondria. The apparent dissociation constants of the binding of thermostable 32P-labeled MAP2 and tau are 0.9 +/- 0.04 x 10(-7) and 3.8 +/- 0.7 x 10(-7) M, respectively. 32P-labeled MAP2 and tau bound to the mitochondria can be displaced by phosphorylated, nonradioactive MAP2. The binding parameters of MAP2 prepared without heat treatment and those of the thermostable MAP2 were of the same order of magnitude. Microtubule-binding and projection domains of MAP2 were obtained by chymotryptic digestion of rat brain microtubules (Vallee, Proc. Natl. Acad. Sci. USA, 77:3206-3210, 1980). Displacement studies with these two domains show that MAP2 bound to mitochondria can be displaced by the microtubule-binding domain, whereas the projection domain does not displace MAP2. The two domains of MAP2 bind to the mitochondria with similar affinity constants; however, the Bmax for the projection domain was 10 times and 35 times lower than the Bmax of the binding of the intact MAP2 and the microtubule-binding domain, respectively. Chymotryptic digestion of MAP2 bound to the mitochondria yielded peptide fragments with mo...Continue Reading

References

Jun 30, 1975·Annals of the New York Academy of Sciences·D S SmithB F Cameron
Feb 1, 1977·The Journal of Cell Biology·P SherlineL S Jacobs
Aug 15, 1977·European Journal of Biochemistry·A FellousJ Nunez
Jan 1, 1987·Cell Motility and the Cytoskeleton·S C Papasozomenos, L I Binder
Apr 1, 1989·The Biochemical Journal·G Wiche
Dec 1, 1986·The Journal of Cell Biology·D G Drubin, M W Kirschner
Nov 13, 1987·Biochemical and Biophysical Research Communications·G FlynnD L Purich
Dec 16, 1987·Biochemical and Biophysical Research Communications·A RendonV Jancsik
Oct 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·U Z LittauerH Ponstingl
Jan 1, 1986·The Journal of Cell Biology·I PengM M Black
Jan 1, 1986·Annual Review of Cell Biology·J B Olmsted
Nov 12, 1987·Nature·B M Paschal, R B Vallee
Dec 16, 1985·European Journal of Biochemistry·L SerranoJ Avila
Jan 2, 1986·European Journal of Biochemistry·M A HernándezJ M Andreu
Mar 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·M L ShelanskiC R Cantor
Jun 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·R Vallee
Sep 1, 1980·Analytical Biochemistry·P J Munson, D Rodbard
Sep 1, 1981·The Journal of Cell Biology·R B ValleeW E Theurkauf
Jan 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·E H Ball, S J Singer
Sep 1, 1982·Journal of Ultrastructure Research·W A Voter, H P Erickson

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Citations

Jan 1, 1994·Cell Motility and the Cytoskeleton·A JellaliA Rendon
Sep 1, 1991·Journal of Neuroscience Research·C W ScottC B Caputo
Dec 1, 1992·Journal of Neuroscience Research·G V Johnson, R S Jope
Sep 1, 1992·Mutation Research·P E Thorsness
Apr 1, 1993·The Journal of Cell Biology·Y WangL I Binder
Nov 1, 1992·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·J L VayssièreB Croizat
May 18, 2021·Frontiers in Cell and Developmental Biology·Eugene BokJaekwang Kim
Nov 21, 2021·Experimental & Molecular Medicine·Yohei TomitaZhongjie Fu

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