PMID: 1194857Dec 1, 1975Paper

Binding of monomeric immunoglobulins to Fc receptors of mouse macrophages

The Journal of Experimental Medicine
J C Unkeless, H N Eisen

Abstract

The binding properties of surface receptors of immunoglobulins on mouse macrophages were studied with mouse myeloma proteins and normal peritoneal macrophages, thioglycollate-stimulated macrophages, and a macrophage cell line, P388D1. Primary cultures of mouse embryo fibroblasts served as controls. IgG2a proteins were bound strongly;IgG2b was bound weakly (one-twentieth as well as IgG2a);IgM, IgA, and IgG1 were not bound significantly. The number of binding sites per cell for IgG2a was 4 X 10(5) for thioglycollate-stimulated cells and 1 X 10(5) for normal and P388D1 cells. Binding was exothermal: with decreasing temperature the equilibrium (association) constants increased and dissociation rate constants decreased (at 37degreesC the respective values were 2 X 10(7) M-1 and 0.26 min-1, the latter value corresponds to a half time for dissociation of 2.6 min). From the rapidity of association and dissociation, it appears that the surface of the macrophage is in a dynamic equilibrium with IgG2a molecules in the cell's immediate microenvironment. The receptors for IgG2a are clearly specific for determinants in the immunoglobulin constant domain: two IgG2a proteins with greatly different isoelectric points (determined by isoelectric ...Continue Reading

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Citations

Jun 1, 1977·Arthritis and Rheumatism·W P ArendG A Starkebaum
Jan 1, 1984·Folia Microbiologica·L Fornůsek, V Vĕtvicka
Jan 1, 1979·Folia Microbiologica·M Ferencík, J Stefanovic
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Dec 31, 1990·Journal of Immunological Methods·T MatsumotoJ C Cyong
Sep 27, 1993·Journal of Immunological Methods·L ShapiraW A Soskolne
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Mar 1, 1993·Developmental and Comparative Immunology·C CauxJ C Monier
Sep 1, 1978·Immunochemistry·J L Winkelhake
Jun 1, 1981·Molecular Immunology·R C SiegelR E Cathou
Nov 1, 1983·Molecular Immunology·D M SegalJ A Titus
Mar 1, 1985·Molecular Immunology·D R Burton
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Jan 1, 1992·Molecular Immunology·J LundG Winter
Apr 1, 1992·Journal of Reproductive Immunology·R A BronsonH B Fleit
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Jan 15, 2011·Analytical Chemistry·Heping YanXiangqun Zeng
Sep 13, 2003·Kidney International·Takayuki FujiiTakashi Saito
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Feb 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·J A HardinJ M Carboni
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Mar 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·S SchreiberS L Perkins
Dec 19, 1995·Proceedings of the National Academy of Sciences of the United States of America·J T HutchinsP Ray
Dec 1, 1981·The Journal of Cell Biology·S B HorwitzO M Rosen

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