Binding of nuclear factors to the 5'-interferon consensus sequence of the HLA-A2 class I gene

Experimental Cell Research
P Le BouteillerF H Ruddle

Abstract

To investigate the regulatory role of the conserved interferon consensus sequence (ICS) found in the 5' flanking region of HLA class I genes, we studied the binding of nuclear proteins to the ICS of HLA-A2 gene (ICS-A2) by the gel shift assay. Nuclear extracts from several human cell lines expressing different levels of surface class I molecules reveal an ICS-A2-protein complex of similar mobility, the amount of which varies in a cell type-dependent manner. In some cell lines, interferon-gamma treatment decreased the level of this complex. The overlapping enhancer A element also competes for the formation of this ICS-A2-protein complex. Footprinting and methylation interference analyses demonstrate that nuclear protein(s) protect specific sequences within the ICS-A2 element, suggesting that these protein(s) may represent interferon-sensitive transcription factors.

References

Nov 1, 1977·Cancer·G M BrodeurM N Goldstein
Aug 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·Y ShirayoshiK Ozato
Apr 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·K OzatoB M Orrison
Mar 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·H G BurgertS Kvist
Feb 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·A S Baldwin, P A Sharp
Oct 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·J A BarbosaF H Ruddle
Jan 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·U Siebenlist, W Gilbert

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