Binding of pertussis toxin to eucaryotic cells and glycoproteins.

Infection and Immunity
M H WitvlietC R Manclark

Abstract

The binding of pertussis toxin and its subunits to cell surface receptors and purified glycoproteins was examined. The interaction of pertussis toxin with components of two variant Chinese hamster ovary (CHO) cell lines was studied. These cell lines are deficient in either sialic acid residues (LEC 2) or sialic acid and galactose residues (LEC 8) on cell surface macromolecules. The binding of pertussis toxin to components of these cells differed from the binding of the toxin to wild-type components. Although the toxin bound to a 165,000-dalton glycoprotein found in N-octylglucoside extracts of wild-type cells, it did not bind to components found in extracts of LEC 2 cells. In contrast, the toxin bound to components found in extracts of LEC 8 cells, which are variant cells that contain increased amounts of terminal N-acetylglucosamine residues on cell surface macromolecules. These results suggest that the receptor for pertussis toxin on CHO cells contains terminal acetamido-containing sugars. The cytopathic effect of the toxin on both types of variant cells was much reduced compared with its effects on wild-type cells. Thus, optimal functional binding of pertussis toxin appears to require a complete sialyllactosamine (NeuAc----G...Continue Reading

References

Jun 1, 1976·The Journal of Experimental Medicine·S I Morse, J H Morse
Jul 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·A NicosiaR Rappuoli
Jan 1, 1987·Annual Review of Biochemistry·A G Gilman
Jan 1, 1987·Annual Review of Cell Biology·G R Martin, R Timpl
Dec 1, 1974·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·P K Nakane, A Kawaoi
Mar 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·J K DzanduG E Wise
Jan 1, 1982·International Review of Cytology·E B Briles
Jan 1, 1983·Methods in Enzymology·H Furthmayr, V T Marchesi
May 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·T Katada, M Ui

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Citations

Oct 24, 2003·Infection and Immunity·Nicholas H CarbonettiZoe E V Worthington
Jul 20, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Cécile CassanRoland S Liblau
Jan 19, 2008·Cellular Microbiology·Roger D Plaut, Nicholas H Carbonetti
Apr 25, 2012·Medical Microbiology and Immunology·Christina BacheThomas Montag
Sep 4, 2014·Expert Review of Vaccines·Anja SeubertMariagrazia Pizza
Nov 26, 2002·The Journal of Biological Chemistry·Alan S CrossSimeon E Goldblum
Jan 24, 2007·Infection and Immunity·Nicholas H CarbonettiVictor I Ayala
Jun 29, 2011·FEMS Immunology and Medical Microbiology·Zoë E V WorthingtonNicholas H Carbonetti
Sep 24, 2015·Pathogens and Disease·Nicholas H Carbonetti
Sep 1, 1994·Nature Structural Biology·P E SteinR J Read
Nov 15, 2003·American Journal of Physiology. Heart and Circulatory Physiology·Jeffrey J OlearczykRandy S Sprague
Oct 14, 2005·Expert Review of Vaccines·Fabrice GodfroidJan Poolman
Oct 9, 2003·Applied and Environmental Microbiology·John A BogdanM S Blake
Sep 9, 2011·Molecular Biotechnology·Shirin Tarahomjoo
Jul 15, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Maria NassoClara Maria Ausiello
Feb 13, 2016·Frontiers in Microbiology·Ana do ValeSandra Sousa
Apr 5, 2002·Microbiology·Franco D MenozziDominique Raze
Oct 3, 2012·Infection and Immunity·Carey E ConnellyNicholas H Carbonetti
Jul 3, 2016·Pathogens and Disease·Roger D PlautNicholas H Carbonetti
Oct 15, 2003·FEMS Immunology and Medical Microbiology·Serhan SakaryaSelcan Oncu
Sep 1, 2017·Human Vaccines & Immunotherapeutics·Marieke HoonakkerCoenraad Hendriksen
Jan 27, 2019·Beilstein Journal of Organic Chemistry·Katharina KolbeThisbe K Lindhorst
Mar 17, 2019·International Journal of Molecular Sciences·Jowita Nowakowska-GołackaMonika Słomińska-Wojewódzka
Jan 1, 1996·Trends in Cell Biology·A L Kolodkin
Sep 1, 1991·Infection and Immunity·T M FinnJ J Mekalanos
Jun 1, 1994·Infection and Immunity·G D ArmstrongL D Heerze
Jun 24, 2016·Microbiology Spectrum·Christian Gonzalez-RiveraPeter J Christie
Dec 1, 1992·Infection and Immunity·M H WitvlietJ T Poolman
Jan 1, 1993·Infection and Immunity·S Z Hausman, D L Burns

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