Binding of phytopolyphenol piceatannol disrupts β/γ subunit interactions and rate-limiting step of steady-state rotational catalysis in Escherichia coli F1-ATPase.

The Journal of Biological Chemistry
Mizuki SekiyaMasamitsu Futai

Abstract

In observations of single molecule behavior under V(max) conditions with minimal load, the F(1) sector of the ATP synthase (F-ATPase) rotates through continuous cycles of catalytic dwells (∼0.2 ms) and 120° rotation steps (∼0.6 ms). We previously established that the rate-limiting transition step occurs during the catalytic dwell at the initiation of the 120° rotation. Here, we use the phytopolyphenol, piceatannol, which binds to a pocket formed by contributions from α and β stator subunits and the carboxyl-terminal region of the rotor γ subunit. Piceatannol did not interfere with the movement through the 120° rotation step, but caused increased duration of the catalytic dwell. The duration time of the intrinsic inhibited state of F(1) also became significantly longer with piceatannol. All of the beads rotated at a lower rate in the presence of saturating piceatannol, indicating that the inhibitor stays bound throughout the rotational catalytic cycle. The Arrhenius plot of the temperature dependence of the reciprocal of the duration of the catalytic dwell (catalytic rate) indicated significantly increased activation energy of the rate-limiting step to trigger the 120° rotation. The activation energy was further increased by com...Continue Reading

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Citations

Jan 14, 2016·Journal of Agricultural and Food Chemistry·Mohamed Ali SeyedKavitha Vijayaraghavan
Jul 15, 2015·Evidence-based Complementary and Alternative Medicine : ECAM·Lara GibelliniMarcello Pinti
Mar 2, 2017·International Journal of Biological Macromolecules·Mizuki SekiyaMayumi Nakanishi-Matsui
May 16, 2018·Proceedings of the National Academy of Sciences of the United States of America·James L MartinWayne D Frasch
Sep 18, 2014·The Journal of Biological Chemistry·Mayumi Nakanishi-MatsuiMasamitsu Futai
Feb 27, 2013·IUBMB Life·Mayumi Nakanishi-MatsuiMasamitsu Futai

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