Binding of plasma proteins to titanium dioxide nanotubes with different diameters

International Journal of Nanomedicine
Mukta KulkarniAleš Iglič

Abstract

Titanium and titanium alloys are considered to be one of the most applicable materials in medical devices because of their suitable properties, most importantly high corrosion resistance and the specific combination of strength with biocompatibility. In order to improve the biocompatibility of titanium surfaces, the current report initially focuses on specifying the topography of titanium dioxide (TiO2) nanotubes (NTs) by electrochemical anodization. The zeta potential (ζ-potential) of NTs showed a negative value and confirmed the agreement between the measured and theoretically predicted dependence of ζ-potential on salt concentration, whereby the absolute value of ζ-potential diminished with increasing salt concentrations. We investigated binding of various plasma proteins with different sizes and charges using the bicinchoninic acid assay and immunofluorescence microscopy. Results showed effective and comparatively higher protein binding to NTs with 100 nm diameters (compared to 50 or 15 nm). We also showed a dose-dependent effect of serum amyloid A protein binding to NTs. These results and theoretical calculations of total available surface area for binding of proteins indicate that the largest surface area (also considerin...Continue Reading

Citations

Sep 24, 2015·Journal of the Mechanical Behavior of Biomedical Materials·S DattaN Sultana
Oct 16, 2015·Dental Materials : Official Publication of the Academy of Dental Materials·Krunal NarendrakumarAleš Iglič
Jan 11, 2016·Journal of Biomedical Materials Research. Part a·Ajda FlaškerSnezna Sodin-Semrl
Jun 18, 2020·International Journal of Nanomedicine·Jinghong YangYan Wang
Apr 4, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Metka BenčinaIta Junkar
Mar 23, 2021·Nanotechnology·Erna JiaZhaohui Su
May 5, 2021·Drug Delivery and Translational Research·Kun WangPeng Li
Jun 10, 2019·ACS Biomaterials Science & Engineering·Jialing WuYan Wang
Apr 14, 2020·ACS Omega·Ita JunkarAleš Iglič

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Methods Mentioned

BETA
phage display
protein assay

Software Mentioned

Nikon
ImageJ

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