Binding of Pseudomonas aeruginosa to neutral glycosphingolipids of rabbit corneal epithelium.

Infection and Immunity
N PanjwaniJ Baum

Abstract

35S-labeled Pseudomonas aeruginosa isolates were shown to bind to neutral glycosphingolipids (NGSLs) of rabbit corneal epithelia in culture by a thin-layer chromatogram overlay procedure. The lipids of the corneal epithelial cells grown in culture were extracted and partitioned into a chloroform-rich lower phase containing NGSLs and an aqueous upper phase containing gangliosides. By using a dot-blot assay, at least six times more radiolabeled P. aeruginosa isolates were shown to bind to the lipids in the lower phase compared with those in the upper phase. Thin-layer chromatography of the lower-phase lipids followed by staining with an orcinol spray revealed at least 10 NGSL components and several fast-migrating, nonglycosylated neutral lipid components (including cholesterol). 35S-labeled P. aeruginosa was shown to bind to NGSL components 1, 2, 5, 6, and 9. P. aeruginosa-reactive NGSL components 6 and 9 migrated with chromatographic mobilities similar to those of the standards ceramide trihexoside (CT) and ceramide monohexoside, respectively. Components 1 and 2 migrated slightly ahead of asialo GM1, and component 5 migrated faster than globoside but slower than CT. Among the various standards tested, P. aeruginosa bound to asia...Continue Reading

References

Jan 1, 1975·Annual Review of Microbiology·R J Gibbons, J V Houte
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Citations

Jan 10, 1998·Infection and Immunity·H LeherJ Y Niederkorn
Aug 1, 1992·Infection and Immunity·N PanjwaniT Zaidi
Jan 1, 1995·Infection and Immunity·Z Zhao, N Panjwani
Jan 1, 1997·Contact Lens & Anterior Eye : the Journal of the British Contact Lens Association·J Dart

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