PMID: 490630Aug 1, 1979Paper

Binding of radioactively labeled saxitoxin to the squid giant axon

The Journal of Membrane Biology
G R StrichartzJ M Ritchie

Abstract

The binding of saxitoxin, a specific inhibitor of the sodium conductance in excitable membranes, has been measured in giant axons from the squid, Loligo pealei. Binding was studied by labeling saxitoxin with tritium, using a solvent-exchange technique, and measuring the toxin uptake by liquid scintillation counting. Total toxin binding is the sum of a saturable, hyperbolic binding component, with a dissociation constant at 2--4 degrees C of 4.3 +/- 1.7 nM (mean SE), and a linear, nonsaturable component. The density of saturable binding sites is 166 +/- 20.4 micrometers-2. From this density and published values of the maximum sodium conductance, the conductance per toxin site is estimated to be about 7 pS, assuming sequential activation and inactivation processes (F. Bezanilla & C.M. Armstrong, 1977, J. Gen. Physiol. 70:549). This single site conductance value of 7 pS is in close aggreement with estimates of the conductance of one open sodium channel from measurements of gating currents and of noise on squid giant axons and is consistent with the hypothesis that one saxitoxin molecule binds to one sodium channel.

References

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Citations

Jan 1, 1985·The Journal of Membrane Biology·F Bezanilla
Feb 1, 1990·Trends in Neurosciences·J A BlackS G Waxman
Feb 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·S G WaxmanJ M Ritchie
Apr 1, 1984·The Journal of Cell Biology·G R StrichartzK H Pfenninger
Jan 1, 1985·The Journal of General Physiology·J R StimersR E Taylor
Nov 1, 1985·The Journal of General Physiology·G K Wang, G Strichartz
Jan 1, 1990·Neurochemistry International·A K Dwivedy
Jan 1, 1986·Annals of the New York Academy of Sciences·J M Ritchie
Aug 27, 2013·Trends in Pharmacological Sciences·Gavin E Jarvis, Andrew J Thompson
Apr 1, 1985·Circulation Research·H A FozzardJ C Makielski
Aug 22, 1984·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·R D Keynes, J M Ritchie
May 29, 2007·American Journal of Physiology. Heart and Circulatory Physiology·Manuel F NavedoLuis F Santana

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