Binding of salivary agglutinin to IgA

The Biochemical Journal
A J M LigtenbergA V Nieuw Amerongen

Abstract

SAG (salivary agglutinin), which is identical to gp-340 (glycoprotein-340) from the lung, is encoded by DMBT1 (deleted in malignant brain tumours 1). It is a member of the SRCR (scavenger receptor cysteine-rich) superfamily and contains 14 SRCR domains, 13 of which are highly similar. SAG in saliva is partially complexed with IgA, which may be necessary for bacterial binding. The goal of the present study was to characterize the binding of purified SAG to IgA. SAG binds to a variety of proteins, including serum and secretory IgA, alkaline phosphatase-conjugated IgGs originating from rabbit, goat, swine and mouse, and lactoferrin and albumin. Binding of IgA to SAG is calcium dependent and is inhibited by 0.5 M KCl, suggesting that electrostatic interactions are involved. Binding of IgA was destroyed after reduction of SAG, suggesting that the protein moiety is involved in binding. To pinpoint further the binding domain for IgA on SAG, a number of consensus-based peptides of the SRCR domains and SRCR interspersed domains were designed and synthesized. ELISA binding studies with IgA indicated that only one of the peptides tested, comprising amino acids 18-33 (QGRVEVLYRGSWGTVC) of the 109-amino-acid SRCR domain, exhibited binding t...Continue Reading

References

Jun 1, 1990·Infection and Immunity·R J Lamont, B Rosan
Jun 15, 1983·European Journal of Biochemistry·T Ericson, J Rundegren
Jan 1, 1994·Trends in Biochemical Sciences·D ResnickM Krieger
Feb 1, 1994·Immunology Today·U HolmskovJ C Jensenius
Sep 15, 1999·Proceedings of the National Academy of Sciences of the United States of America·U HolmskovK Skjodt
Mar 4, 2000·Antonie van Leeuwenhoek·A J LigtenbergA V Nieuw Amerongen
Feb 24, 2001·Nature·M KristiansenS K Moestrup
Mar 7, 2001·European Journal of Biochemistry·G T NagleK W Li
Mar 1, 2003·Journal of Microbiological Methods·J A BoschA V Nieuw Amerongen
Apr 12, 2003·AIDS Research and Human Retroviruses·Zhiwei WuDaniel Malamud
Jul 23, 2003·American Journal of Physiology. Lung Cellular and Molecular Physiology·Kevan L HartshornUffe Holmskov

❮ Previous
Next ❯

Citations

Jan 19, 2008·American Journal of Physiology. Gastrointestinal and Liver Physiology·Robert C De LisleDonna Ziemer
Jun 15, 2007·BMC Infectious Diseases·Anette JonassonNicklas Strömberg
Jan 1, 2010·International Journal of Molecular Sciences·Antoon J M LigtenbergEnno C I Veerman
Aug 25, 2009·The Journal of Thoracic and Cardiovascular Surgery·Hanna MüllerJan Mollenhauer
Mar 26, 2016·Frontiers in Immunology·Martin Parnov Reichhardt, Seppo Meri
Jan 6, 2009·Oral Microbiology and Immunology·M R WhiteK L Hartshorn
May 3, 2008·Molecular Microbiology·Andrew M EdwardsGuido Grandi
Nov 25, 2011·European Journal of Oral Sciences·Mikael SonessonClaes Wickström
Jan 22, 2013·European Journal of Oral Sciences·Floris J BikkerEnno C I Veerman
Jul 15, 2015·Analytical and Bioanalytical Chemistry·Gianluca AdornettoDanila Moscone
Apr 27, 2010·Innate Immunity·Jens MadsenUffe Holmskov
Dec 15, 2017·Journal of Cellular Biochemistry·María Lorena RoldánPatricia Estela Marini
Apr 2, 2017·Immunogenetics·Floris J BikkerJan Mollenhauer
Jul 3, 2017·Molecular Immunology·M P ReichhardtS Meri

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.