PMID: 9436994Mar 14, 1998Paper

Binding of signal recognition particle gives ribosome/nascent chain complexes a competitive advantage in endoplasmic reticulum membrane interaction

Molecular Biology of the Cell
A NeuhofT A Rapoport

Abstract

Most secretory and membrane proteins are sorted by signal sequences to the endoplasmic reticulum (ER) membrane early during their synthesis. Targeting of the ribosome-nascent chain complex (RNC) involves the binding of the signal sequence to the signal recognition particle (SRP), followed by an interaction of ribosome-bound SRP with the SRP receptor. However, ribosomes can also independently bind to the ER translocation channel formed by the Sec61p complex. To explain the specificity of membrane targeting, it has therefore been proposed that nascent polypeptide-associated complex functions as a cytosolic inhibitor of signal sequence- and SRP-independent ribosome binding to the ER membrane. We report here that SRP-independent binding of RNCs to the ER membrane can occur in the presence of all cytosolic factors, including nascent polypeptide-associated complex. Nontranslating ribosomes competitively inhibit SRP-independent membrane binding of RNCs but have no effect when SRP is bound to the RNCs. The protective effect of SRP against ribosome competition depends on a functional signal sequence in the nascent chain and is also observed with reconstituted proteoliposomes containing only the Sec61p complex and the SRP receptor. We co...Continue Reading

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Citations

Mar 21, 2000·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·K Mihara
Oct 8, 1999·Molecular Biology of the Cell·U Fünfschilling, S Rospert
Nov 18, 2003·Molecular Biology of the Cell·Sylvie Le GallTom Rapoport
Jul 11, 2006·Molecular Biology of the Cell·Julia Schaletzky, Tom A Rapoport
Dec 28, 1999·Annual Review of Cell and Developmental Biology·A E Johnson, M A van Waes
Sep 13, 2005·Annual Review of Microbiology·Mechthild PohlschröderAlex Haddad
Nov 12, 2009·Journal of Cell Science·Benedict C S CrossEileithyia Swanton
Nov 13, 1998·Proceedings of the National Academy of Sciences of the United States of America·I MöllerB Lauring
Mar 1, 2008·Molecular Membrane Biology·Beáta LizákGábor Bánhegyi
Oct 19, 2000·The Journal of Cell Biology·S KanajiK Mihara
Apr 11, 2015·Science·Günter KramerBernd Bukau
Sep 10, 2008·Biochimica Et Biophysica Acta·Kai-Uwe KaliesEnno Hartmann
Dec 27, 2011·Cell·Sichen Shao, Ramanujan S Hegde
Feb 7, 2015·PloS One·Marie-Luise Kaiser, Karin Römisch
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Nov 30, 2010·Cell·Yoko ShibataTom A Rapoport

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