PMID: 20632933Jul 17, 2010Paper

Binding of sterols affects membrane functioning and sphingolipid composition in wheat roots

Biochemistry. Biokhimii︠a︡
Yu N ValitovaFarida Minibayeva

Abstract

The present work was devoted to the exploration of the role of sterols in the functioning of membranes in root cells. Membrane characteristics and composition of the membrane lipids in the roots of wheat (Triticum aestivum L.) seedlings treated with exogenous cholesterol and antibiotic nystatin, which specifically binds with endogenous sterols, were analyzed. Cholesterol caused a fall of membrane potential, acidification of the incubation medium, decrease in potassium leakage of roots, and increase in the level of exogenous superoxide radical. Similarly to cholesterol, the application of nystatin also induced the depolarization of the plasma membrane, but in contrast with cholesterol it was accompanied by alkalinization of the incubation medium and decrease in the level of exogenous superoxide radical. Analysis of membrane lipids showed that following nystatin treatment the total sterol content in roots did not change, while the level of complex sphingolipids represented mainly by glycoceramides became higher. Using mass spectrometry with electrospray ionization ((+)ESI-MS) for the analysis of the glycoceramide composition, we showed that nystatin induced changes in the ratios of molecular species of glycoceramides. It was sugg...Continue Reading

References

Aug 1, 1968·The Journal of General Physiology·T E Andreoli, M Monahan
Apr 18, 2000·Journal of Mass Spectrometry : JMS·M C SullardsJ Adams
Oct 12, 2000·The Journal of Biological Chemistry·L J Siskind, M Colombini
Nov 1, 2002·The Biochemical Journal·Wim J van BlitterswijkJannie Borst
Jan 30, 2004·Biochemical Society Transactions·C J Fielding, P E Fielding
Mar 24, 2004·Annals of Botany·Teresa M DunnJohnathan A Napier
Apr 24, 2004·Antimicrobial Agents and Chemotherapy·Kasturi MukhopadhyayRajendra Prasad
Aug 31, 2004·Anais Da Academia Brasileira De Ciências·Senitiroh Hakomori
Apr 22, 2005·Biological Chemistry·Helen E TownleyChristopher J Leaver
Jun 14, 2005·Trends in Plant Science·Stephen W MartinJulia M Davies
Apr 21, 2006·Nature Reviews. Molecular Cell Biology·John F Hancock
May 9, 2006·Antioxidants & Redox Signaling·Gabriella LeonarduzziFiorella Biasi
Dec 27, 2008·Biochimica Et Biophysica Acta·Dong DengGuo-jiang Zhang
Feb 17, 2009·The Biochemical Journal·Laurence BessueilleVincent Bulone

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Citations

May 6, 2014·Doklady. Biochemistry and Biophysics·A G SulkarnayevaF V Minibayeva
Sep 30, 2016·Biochemistry. Biokhimii︠a︡·J N ValitovaF V Minibayeva
Jun 21, 2021·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Maksim Steshin, Toshiki Ishikawa

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