Binding of SV40's Viral Capsid Protein VP1 to Its Glycosphingolipid Receptor GM1 Induces Negative Membrane Curvature: A Molecular Dynamics Study

Langmuir : the ACS Journal of Surfaces and Colloids
Raisa KociurzynskiVolker Knecht

Abstract

The binding of the pentameric capsid protein VP1 of simian virus 40 to its glycosphingolipid receptor GM1 is a key step for the entry of the virus into the host cell. Recent experimental studies have shown that the interaction of variants of soluble VP1 pentamers with giant unilamellar vesicles composed of GM1, DOPC, and cholesterol leads to the formation of tubular membrane invaginations to the inside of the vesicles, mimicking the initial steps of endocytosis. We have used coarse-grained and atomistic molecular dynamics (MD) simulations to study the interaction of VP1 with GM1/DOPC/cholesterol bilayers. In the presence of one VP1 protein, we monitor the formation of small local negative curvature and membrane thinning at the protein binding site as well as reduction of area per lipid. These membrane deformations are also observed under cholesterol-free conditions. However, here, the number of GM1 molecules attached to the VP1 binding pockets increases. The membrane curvature is slightly increased for asymmetric GM1 distribution that mimics conditions in vivo, compared to symmetric GM1 distributions which are often applied in experiments. Slightly smaller inward curvature was observed in atomistic control simulations. Binding ...Continue Reading

References

Nov 28, 1991·Nature·R C LiddingtonS C Harrison
Dec 15, 1967·Science·D M Derry, L S Wolfe
Aug 25, 1995·Journal of Molecular Biology·R G ZhangG G Shipley
Jul 18, 2002·European Biophysics Journal : EBJ·Manuela MenkeAndreas Janshoff
Jan 1, 1962·Cold Spring Harbor Symposia on Quantitative Biology·D L CASPAR, A KLUG
Jan 26, 2005·The Journal of Cell Biology·Matthew KirkhamRobert G Parton
Jul 28, 2005·Biochimica Et Biophysica Acta·Matthew Kirkham, Robert G Parton
Sep 20, 2006·Glycobiology·Sandro SonninoAlessandro Prinetti
Apr 14, 2007·Journal of the American Chemical Society·Jinjun ShiPaul S Cremer
Jun 16, 2007·The Journal of Physical Chemistry. B·Siewert J MarrinkAlex H de Vries
Nov 30, 2007·Nature·Winfried RömerLudger Johannes
Jan 19, 2008·Science·Huichen FengPatrick S Moore
Mar 21, 2008·Proceedings of the National Academy of Sciences of the United States of America·Ursula NeuThilo Stehle
Dec 22, 2009·Nature Cell Biology·Helge EwersLudger Johannes
Apr 7, 2011·Current Opinion in Cell Biology·Kirsten SandvigBo van Deurs
Apr 13, 2012·The Journal of Physical Chemistry. B·Kenichi MoriTyuji Hoshino
May 15, 2013·Korean Journal of Pathology·Minseob EomSoon-Hee Jung
Oct 24, 2013·Journal of Computer-aided Molecular Design·Vytautas GapsysRodolfo Briones
Aug 20, 2014·Proceedings of the National Academy of Sciences of the United States of America·Thorsten EierhoffWinfried Römer
Nov 27, 2014·Langmuir : the ACS Journal of Surfaces and Colloids·Ipsita Basu, Chaitali Mukhopadhyay
Aug 8, 2015·Langmuir : the ACS Journal of Surfaces and Colloids·Huijiao SunWeihai Fang
Nov 18, 2015·Journal of Chemical Theory and Computation·Tsjerk A WassenaarSiewert J Marrink
Feb 11, 2014·Journal of Chemical Theory and Computation·Tsjerk A WassenaarD Peter Tieleman
Mar 12, 2013·Journal of Chemical Theory and Computation·César A LópezSiewert J Marrink
Dec 8, 2009·Journal of Chemical Theory and Computation·Cesar A LópezSiewert J Marrink
Sep 8, 2009·Journal of Chemical Theory and Computation·Xavier PerioleMarco A Ceruso
May 1, 2008·Journal of Chemical Theory and Computation·Luca MonticelliSiewert-Jan Marrink
Jul 31, 2016·Journal of Molecular Graphics & Modelling·Akshay SridharAshok Kumar Dasmahapatra
Dec 13, 2016·ACS Nano·Weria PezeshkianJulian C Shillcock
Nov 11, 2017·FEBS Open Bio·Weria PezeshkianJohn H Ipsen
Nov 11, 2017·Journal of Physics. Condensed Matter : an Institute of Physics Journal·Ricardo de Oliveira Dos Santos SoaresAntonio Caliri

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Citations

Aug 10, 2019·Frontiers in Molecular Biosciences·Weria PezeshkianJohn H Ipsen
Aug 15, 2021·Glycoconjugate Journal·Fulvio ReggioriEeva-Liisa Eskelinen

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