Binding of the bioflavonoid robinetin with model membranes and hemoglobin: Inhibition of lipid peroxidation and protein glycosylation

Journal of Photochemistry and Photobiology. B, Biology
Sudip ChaudhuriPradeep K Sengupta

Abstract

Recent years have witnessed burgeoning interest in plant flavonoids as novel therapeutic drugs targeting cellular membranes and proteins. Motivated by this scenario, we explored the binding of robinetin (3,7,3',4',5'-pentahydroxyflavone, a bioflavonoid with remarkable 'two color' intrinsic fluorescence properties), with egg yolk phosphatidylcholine (EYPC) liposomes and normal human hemoglobin (HbA), using steady state and time resolved fluorescence spectroscopy. Distinctive fluorescence signatures obtained for robinetin indicate its partitioning (K(p)=8.65x10(4)) into the hydrophobic core of the membrane lipid bilayer. HbA-robinetin interaction was examined using both robinetin fluorescence and flavonoid-induced quenching of the protein tryptophan fluorescence. Specific interaction with HbA was confirmed from three lines of evidence: (a) bimolecular quenching constant K(q)>diffusion controlled limit; (b) closely matched values of Stern-Volmer quenching constant and binding constant; (c) tau(0)/tau=1 (where tau(0) and tau are the unquenched and quenched tryptophan fluorescence lifetimes, respectively). Absorption spectrophotometric assays reveal that robinetin inhibits EYPC membrane lipid peroxidation and HbA glycosylation with ...Continue Reading

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Citations

May 25, 2010·The Protein Journal·Qiu-Hua ZhouYan-Qing Wang
May 30, 2012·Journal of Photochemistry and Photobiology. B, Biology·Yan-Qing Wang, Hong-Mei Zhang
Jul 20, 2010·Journal of Photochemistry and Photobiology. B, Biology·Yan-Qing WangBo-Ping Tang
Aug 25, 2015·The Journal of Physical Chemistry. B·Jitendriya Swain, Ashok Kumar Mishra
Dec 9, 2014·Natural Product Research·Maria Paola GermanòAntonio Rapisarda
Jan 30, 2014·Biochimica Et Biophysica Acta·Yury S TarahovskyEugeny N Muzafarov
Nov 26, 2019·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Monalisa Mohapatra, Ashok Kumar Mishra

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