Binding of the CryIVD Toxin of Bacillus thuringiensis subsp. israelensis to Larval Dipteran Midgut Proteins.

Applied and Environmental Microbiology
F FeldmannC Waalwijk

Abstract

Ligand-blotting experiments on dipteran brush border membrane vesicles (BBMVs) showed binding of CryIVD toxin of Bacillus thuringiensis subsp. israelensis to proteins of 148 kDa in Anopheles stephensi and of 78 kDa in Tipula oleracea, both species being susceptible to CryIVD. Binding of CryIVD with BBMVs of A. stephensi resulted in a stronger signal than with BBMVs of T. oleracea. Likewise, larvae of A. stephensi are 10,000-fold more susceptible to the CryIVD toxin than are larvae of T. oleracea. Binding was also found with six proteins ranging in size from 48 to 110 kDa in BBMVs from the lepidopteran species Manduca sexta, but CryIVD was not toxic for M. sexta larvae. No binding of trypsinated CryIVD to BBMV proteins was observed. With the lepidopteran-specific toxin CryIA(b), no binding to dipteran BBMVs was found. Binding of CryIA(b) to nine different BBMV proteins ranging in size from 71 to 240 kDa was observed in M. sexta. The major binding signal was observed with a protein of 240 kDa for CryIA(b).

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Citations

May 15, 2002·Bioscience, Biotechnology, and Biochemistry·Masashi YamagiwaHiroshi Sakai
Jul 22, 2008·Environmental Microbiology·Zachariah Ngalo Otieno-AyayoEitan Ben-Dov
Jan 8, 1999·Journal of Invertebrate Pathology·M Abdul-Rauf, D J Ellar
Nov 1, 2001·Trends in Parasitology·L RegisJ F Charles
Aug 1, 1996·Applied and Environmental Microbiology·C Du, K W Nickerson

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