Binding of the influenza A virus to cell-surface receptors: structures of five hemagglutinin-sialyloligosaccharide complexes determined by X-ray crystallography

Virology
M B EisenD C Wiley

Abstract

The structures of five complexes of the X-31 influenza A (H3N2) virus hemagglutinin with sialyloligosaccharide receptor analogs have been determined from 2.5 to 2.8 A resolution by X-ray crystallography. There is well-defined electron density for three to five saccharides in all five complexes and a striking conformational difference between two linear pentasaccharides with the same composition but different linkage [alpha(2-->6) or alpha(2-->3)] at the terminal sialic acid. The bound position of the terminal sialic acid (NeuAc) is the same in all five complexes and is identical to that reported previously from the study of mono- and trisaccharides. The two oligosaccharides with NeuAc alpha(2-->6)Gal linkages and GlcNAc at the third position have a folded conformation with the GlcNAc doubled back to contact the sialic acid. The pentasaccharide with a terminal NeuAc alpha(2-->3)Gal linkage and GlcNAc at the third position has an extended (not folded) conformation and exits from the opposite side of the binding site than the alpha(2-->6)-linked molecule of the same composition. The difference between the conformation of the pentasaccharide with a 2,6 linkage and the trisaccharide 2,6-sialyllactose suggests that 2,6-sialyllactose ...Continue Reading

References

Jan 1, 1991·Journal of Virology·K A Ryan-Poirier, Y Kawaoka
Jan 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·N K SauterD C Wiley
Sep 30, 1991·Carbohydrate Research·S SabesanJ C Paulson
Apr 20, 1990·Journal of Molecular Biology·W I WeisD C Wiley
Jan 1, 1987·Annual Review of Biochemistry·D C Wiley, J J Skehel
Jun 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·R VlasakP Palese
Aug 2, 1972·Nature: New Biology·C M Brand, J J Skehel
Sep 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·M A MarkwellJ C Paulson
Dec 15, 1994·Structure·D W Rodgers
Feb 1, 1994·Protein Science : a Publication of the Protein Society·E A MerrittW G Hol
Sep 1, 1994·Acta Crystallographica. Section D, Biological Crystallography·UNKNOWN Collaborative Computational Project, Number 4

❮ Previous
Next ❯

Citations

Aug 25, 2010·Glycoconjugate Journal·Karthik ViswanathanRam Sasisekharan
Oct 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Junfeng LiuJohn J Skehel
Aug 8, 2012·Proceedings of the National Academy of Sciences of the United States of America·Christian SiebenAndreas Herrmann
Dec 14, 2012·Proceedings of the National Academy of Sciences of the United States of America·Yi Pu LinJohn W McCauley
Jun 12, 2010·The Journal of Biological Chemistry·Steven J Gamblin, John J Skehel
Jul 27, 2000·Glycobiology·S J StrayG M Air
Jul 20, 1999·Annual Review of Biophysics and Biomolecular Structure·C A BushA Imberty
Oct 19, 2007·Proceedings of the National Academy of Sciences of the United States of America·Qinghua WangJianpeng Ma
Sep 20, 2001·Proceedings of the National Academy of Sciences of the United States of America·Y HaD C Wiley
Jul 16, 2014·Proceedings of the National Academy of Sciences of the United States of America·Patrick J CollinsJohn J Skehel
Jun 4, 2015·Emerging Microbes & Infections·Young-Il KimYoung Ki Choi
Jan 31, 2013·The Journal of Biological Chemistry·Gabriel L HendricksJennifer P Wang
Apr 1, 2006·Glycoconjugate Journal·R J RussellJ J Skehel
Oct 14, 2010·Natural Product Reports·Hu GeHua Su
Mar 17, 2004·Virus Research·N A IlyushinaN V Bovin
Apr 27, 2013·Virus Research·James C Paulson, Robert P de Vries
Jan 6, 2012·Biosensors & Bioelectronics·Emi SuenagaKumar K R Penmetcha
Aug 16, 2008·Carbohydrate Research·Haydn R SinclairRobert A Rastall

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.