Binding of the monoclonal antibody RP215 to immunoglobulin G in metastatic lung adenocarcinomas is correlated with poor prognosis

Histopathology
Yang LiuXiaoyan Qiu

Abstract

Cancer cell-derived immunoglobulin (Ig)G (cancer-IgG) has been found to be involved in the pathogenesis and progression of many cancers, including lung cancer. The aim of the present study was to investigate the relationship between cancer-IgG expression in lung adenocarcinoma (ADC) and clinicopathological characteristics and clinical outcome. Immunohistochemical analysis was performed using an RP215 monoclonal antibody to determine cancer-IgG expression in 140 lung ADC patients. Cell migration and invasion were analysed in A549 cell line after short interfering RNA (siRNA) knockdown of IgG and cell sorting by flow cytometry. Our results show that RP215 immunostaining score is correlated significantly with local invasion (P < 0.05) and tumour differentiation (P < 0.05) in ADC. Moreover, RP215 staining was significantly higher in metastatic tumours than in primary tumours (P < 0.0001). The knockdown of IgG resulted in a reduction of cell migration and invasion. In contrast, RP215-positive cells displayed greater migration and invasion ability than RP215-negative cells. Additionally, a higher RP215 immunostaining score was associated significantly with poor prognosis. RP215 staining is correlated strongly with differentiation, lo...Continue Reading

References

May 23, 2002·The American Journal of Surgical Pathology·Yasushi YatabeTakashi Takahashi
Oct 16, 2002·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Nobuki NakamuraRyohei Katoh
Jul 23, 2003·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Takashi HiranoHarubumi Kato
Nov 2, 2005·Pathology, Research and Practice·Akihiro SuzukiNoriyuki Sato
May 4, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Zhengshan Chen, Jiang Gu
Dec 20, 2007·Applied Immunohistochemistry & Molecular Morphology : AIMM·Aydanur KargiBurçin Tuna
Feb 5, 2008·The International Journal of Biochemistry & Cell Biology·Jing HuangXiaoyan Qiu
Dec 10, 2008·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Jing HuangXiaoyan Qiu
May 2, 2009·Cancer Biomarkers : Section a of Disease Markers·Gregory Lee
Sep 5, 2009·Cancer Biomarkers : Section a of Disease Markers·Gregory Lee, Bixia Ge
Dec 25, 2009·Cellular and Molecular Life Sciences : CMLS·Shuai ZhangXiaoyan Qiu
Nov 10, 2010·Cellular and Molecular Life Sciences : CMLS·Na NiuJiang Gu
Dec 21, 2010·The International Journal of Biochemistry & Cell Biology·Na NiuJiang Gu
Mar 21, 2012·Cancer Immunology, Immunotherapy : CII·Gregory LeeJiang Gu
Jun 16, 2012·CA: a Cancer Journal for Clinicians·Rebecca SiegelElizabeth Ward
Jan 22, 2013·CA: a Cancer Journal for Clinicians·Rebecca SiegelAhmedin Jemal

❮ Previous
Next ❯

Citations

Nov 1, 2015·Journal of Clinical Pathology·Zhengzuo ShengTao Xu
Apr 11, 2017·The International Journal of Biochemistry & Cell Biology·Zhu ZhuBin Wang
Jun 23, 2016·Oncotarget·Meng ShenXiubao Ren
Mar 8, 2018·International Journal of Molecular Medicine·Ziyang JingYue Wang
Nov 23, 2019·Cellular & Molecular Immunology·Zihan WangXiaoyan Qiu
Mar 29, 2019·World Journal of Gastrointestinal Oncology·Zi-Han GengXiao-Yan Qiu
Apr 13, 2021·Frontiers in Immunology·Ming CuiXiaoyan Qiu

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cancer Vaccines

Cancer vaccines are vaccines that either treat existing cancer or prevent development of a cancer.

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Cell Migration in Cancer and Metastasis

Migration of cancer cells into surrounding tissue and the vasculature is an initial step in tumor metastasis. Discover the latest research on cell migration in cancer and metastasis here.