PMID: 2510823Sep 5, 1989Paper

Binding of tissue-type plasminogen activator to lysine, lysine analogues, and fibrin fragments

Biochemistry
G A de MunkJ H Verheijen

Abstract

Human tissue-type plasminogen activator (t-PA) consists of five domains designated (starting from the N-terminus) finger, growth factor, kringle 1, kringle 2, and protease. The binding of t-PA to lysine-Sepharose and aminohexyl-Sepharose was found to require kringle 2. The affinity for binding the lysine derivatives 6-aminohexanoic acid and N-acetyllysine methyl ester was about equal, suggesting that t-PA does not prefer C-terminal lysine residues for binding. Intact t-PA and a variant consisting only of kringle 2 and protease domains were found to bind to fibrin fragment FCB-2, the very fragment that also binds plasminogen and acts as a stimulator of t-PA-catalyzed plasminogen activation. In both cases, binding could completely be inhibited by 6-aminohexanoic acid, pointing to the involvement of a lysine binding site in this interaction. Furthermore, the second site in t-PA involved in interaction with fibrin, presumably the finger, appears to interact with a part of fibrin, different from FCB-2.

References

Jul 1, 1978·The Journal of Experimental Medicine·A Granelli-Piperno, E Reich
Jul 1, 1978·Archives of Biochemistry and Biophysics·R Radcliffe, T Heinze
Sep 1, 1977·Thrombosis Research·S A Cederholm-Williams
Jul 1, 1986·The Journal of Clinical Investigation·A IchinoseK Fujikawa
Jul 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·A J van ZonneveldH Pannekoek
Mar 1, 1988·European Journal of Biochemistry·P J BosmaW Nieuwenhuizen
May 15, 1985·European Journal of Biochemistry·B NorrmanM Rånby
Feb 1, 1988·Analytical Biochemistry·P RauberE Shaw
Sep 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·T NyB Lund
May 1, 1981·Molecular and Cellular Biology·R C Mulligan, P Berg

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Citations

Sep 11, 2004·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Evgenia G VlakhTatiana B Tennikova
Nov 6, 2001·Journal of Biochemical and Biophysical Methods·G Houen
Sep 1, 1993·European Journal of Biochemistry·V FleuryE Anglés-Cano
Feb 1, 1994·European Journal of Biochemistry·P GrailheE Anglés-Cano
Aug 17, 2005·Journal of Thrombosis and Haemostasis : JTH·M W Mosesson
Oct 9, 2015·The Journal of Physical Chemistry. B·Mohammad RehanGirish Sahni
Jul 11, 2012·The Journal of Pharmacy and Pharmacology·Martin Flemmig, Matthias F Melzig
Apr 1, 1992·Archives of Biochemistry and Biophysics·V S DeSerranoF J Castellino
Jul 23, 2003·Journal of Thrombosis and Haemostasis : JTH·M W Mosesson
Dec 17, 1997·Biochemical and Biophysical Research Communications·I W BobbinkP G de Groot
May 23, 1998·Arteriosclerosis, Thrombosis, and Vascular Biology·P H QuaxJ H Verheijen
Jan 26, 2010·Journal of Thrombosis and Haemostasis : JTH·V K LishkoT P Ugarova
Jun 6, 2003·Journal of Neurophysiology·Frank RichterHans-Georg Schaible
Jul 31, 2019·Computational and Structural Biotechnology Journal·Jan MicanJiri Damborsky
Feb 19, 2021·Journal of Stroke·Dmitri NikitinDong-Eog Kim
Oct 27, 1995·The Journal of Biological Chemistry·P BringmannP Donner
Sep 1, 1991·Progress in Cardiovascular Diseases·J HenkinH C Yang

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