PMID: 7513640Apr 15, 1994Paper

Binding of transforming growth factor-beta (TGF-beta) to pregnancy zone protein (PZP). Comparison to the TGF-beta-alpha 2-macroglobulin interaction

European Journal of Biochemistry
A PhilipM D O'Connor-McCourt

Abstract

Pregnancy zone protein (PZP) is quantitatively the most important pregnancy-associated plasma protein and it has strong similarity to alpha 2-macroglobulin. Since alpha 2-macroglobulin is a binding protein for transforming growth factors-beta (TGF-beta), it was of interest to test whether the related protein, PZP, also binds to these growth-regulatory proteins. Using affinity-labelling methods, we demonstrate that PZP binds both TGF-beta 1 and TGF-beta 2 and that the binding characteristics are similar to those of the TGF-beta-alpha 2-macroglobulin interaction. TGF-beta 2 and TGF-beta 1 bind to PZP in a predominantly noncovalent manner in vitro. TGF-beta 1 and TGF-beta 2 bind to both the dimeric and tetrameric forms of PZP. Our studies also indicate that PZP binds TGF-beta 2 with higher affinity than TGF-beta 1. Finally, we demonstrate that PZP inhibits the binding of TGF-beta 1 and TGF-beta 2 to their cell surface receptors. The increased level of PZP during pregnancy may affect the action of TGF-beta by regulating the distribution, clearance and/or general availability of TGF-beta. The preferential binding of TGF-beta 2 over TGF-beta 1 by PZP implies that PZP may differentially regulate the action of TGF-beta 1 and TGF-beta 2.

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Citations

Aug 4, 2010·Journal of Proteome Research·Chengjian TuDaniel C Liebler
Sep 17, 2009·Molecular & Cellular Proteomics : MCP·Stuart A CainCay M Kielty
Apr 5, 2007·Arthritis Research & Therapy·Reuben GobezieDavid M Lee
Aug 17, 2010·Gastroenterology·Naga ChalasaniUNKNOWN Nonalcoholic Steatohepatitis Clinical Research Network
Sep 16, 2015·Reproduction : the Official Journal of the Society for the Study of Fertility·Mohammad AtikuzzamanHeriberto Rodriguez-Martinez
Jan 1, 1997·Placenta·M A Kessler, L A Schuler
Apr 1, 1995·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·A AbrahamianJ H Rockey
Mar 10, 2019·Proceedings of the National Academy of Sciences of the United States of America·Jordan H CaterAmy R Wyatt
Aug 21, 2019·Oxidative Medicine and Cellular Longevity·Jordan H CaterAmy R Wyatt
Mar 22, 2020·Scientific Reports·Hirofumi KashiwagiYoshie Kametani
Jun 17, 2020·Scientific Reports·Daniel Bastida-RuizMarie Cohen

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