PMID: 6169139Jan 1, 1980Paper

Binding of urokinase to plasma proteinase inhibitors

Scandinavian Journal of Clinical and Laboratory Investigation
L HolmbergB Astedt

Abstract

125I-labelled urokinase was incubated with plasma and plasminogen free plasma, and the incubation mixtures were analyzed by agarose gel electrophoresis. Autoradiography demonstrated that non-reacted urokinase remained around the slit and that complex-formation with inhibitors altered the migration and resulted in two bands, a major one and a minor one. Crossed immunoelectrophoresis combined with autoradiography showed that the major band contained a complex between alpha 2-antiplasmin and urokinase. The minor band contained a complex between alpha 2-macroglobulin and urokinase. Also di-isopropylfluorophosphate-inactivated urokinase was bound to alpha 2-macroglobulin but not to alpha 2-antiplasmin. Thus, an intact active site of urokinase is not necessary for complex formation with alpha 2-macroglobulin.

References

Aug 1, 1976·The Biochemical Journal·E Vahtera, U Hamberg
Aug 12, 1976·Biochimica Et Biophysica Acta·L HolmbergB Astedt
Apr 8, 1976·Biochimica Et Biophysica Acta·I Clemmensen, F Christensen
Jul 1, 1968·Clinica Chimica Acta; International Journal of Clinical Chemistry·T R Boyde, I F Pryme
Dec 28, 1971·Biochimica Et Biophysica Acta·J I Thorell, B G Johansson
Oct 1, 1952·Archives of Biochemistry and Biophysics·T ASTRUP, S MULLERTZ
Jun 1, 1962·The American Journal of Physiology·R H PAINTER, A F CHARLES

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Citations

Jan 1, 1986·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·M H Hanlon, I E Liener
Jun 1, 1996·The Journal of Clinical Investigation·P A MenoudA P Sappino
Jan 1, 1986·Critical Reviews in Oncology/hematology·D Collen, H R Lijnen
Aug 1, 1985·Clinical Physiology·L Kaijser, C Sachs
Apr 20, 2021·Frontiers in Cardiovascular Medicine·Jordan R YaronAlexandra R Lucas
Apr 29, 1988·Biochemical and Biophysical Research Communications·T YoshimotoY Inada

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