Binding patterns of homo-peptides on bare magnetic nanoparticles: insights into environmental dependence

Scientific Reports
S A Blank-ShimS Berensmeier

Abstract

Magnetic nanoparticles (MNP) are intensively investigated for applications in nanomedicine, catalysis and biotechnology, where their interaction with peptides and proteins plays an important role. However, the characterisation of the interaction of individual amino acids with MNP remains challenging. Here, we classify the affinity of 20 amino acid homo-hexamers to unmodified iron oxide nanoparticles using peptide arrays in a variety of conditions as a basis to identify and rationally design selectively binding peptides. The choice of buffer system is shown to strongly influence the availability of peptide binding sites on the MNP surface. We find that under certain buffer conditions peptides of different charges can bind the MNP and that the relative strength of the interactions can be modulated by changing the buffer. We further present a model for the competition between the buffer and the MNP's electrostatically binding to the adsorption sites. Thereby, we demonstrate that the charge distribution on the surface can be used to correlate the binding of positively and negatively charged peptides to the MNP. This analysis enables us to engineer the binding of MNP on peptides and contribute to better understand the bio-nano inter...Continue Reading

References

Dec 16, 1998·Journal of Colloid and Interface Science·L GarcellC J Serna
Mar 17, 2004·Nature Biotechnology·Jong Wook HongStephen R Quake
Feb 24, 2006·Applied Microbiology and Biotechnology·Matthias FranzrebOwen R T Thomas
Feb 6, 2007·The Journal of Biological Chemistry·Edgar E KooijmanBen de Kruijff
Mar 21, 2007·Journal of the American Society for Mass Spectrometry·Sarah Y ChangC R Cris Wang
Apr 3, 2007·Journal of the American Chemical Society·Lisa A CapriottiJoel P Schneider
Feb 22, 2008·Bioconjugate Chemistry·Michal BabicEva Syková
Mar 22, 2008·Science·Alexandra NavrotskyJuraj Majzlan
Jun 26, 2009·Journal of the American Chemical Society·Hendrik HeinzRajesh R Naik
Nov 7, 2009·Methods in Enzymology·Helena BlockFrank Schäfer
Nov 17, 2009·ACS Nano·Angana SenpanGregory M Lanza
May 28, 2010·Biotechnology and Bioengineering·Mina OkochiHiroyuki Honda
Aug 3, 2010·Accounts of Chemical Research·Anne ValleeClaire-Marie Pradier
Aug 31, 2010·Analytical Chemistry·Sahar AhmedKamaljit Kaur
Mar 16, 2011·Chemical Reviews·Vivek PolshettiwarJean-Marie Basset
Jul 28, 2009·ACS Nano·Candan Tamerler, Mehmet Sarikaya
Jan 14, 2012·Journal of the American Chemical Society·Julian Schneider, Lucio Colombi Ciacchi
Apr 7, 2012·Chemical Society Reviews·Miriam ColomboWolfgang J Parak
May 25, 2012·ACS Nano·Anton A ShemetovAlyona Sukhanova
Jun 12, 2012·Journal of the American Chemical Society·Albert RimolaPiero Ugliengo
May 31, 2013·Journal of Separation Science·Bohuslav Rittich, Alena Spanová
Dec 18, 2013·Nature Structural & Molecular Biology·Hume StroudSteven E Jacobsen
Mar 13, 2014·Environmental Toxicology and Chemistry·Sabine Goldberg
Apr 4, 2014·Langmuir : the ACS Journal of Surfaces and Colloids·Anna Gitelman, Hanna Rapaport
Jun 18, 2014·Journal of Chemical Information and Modeling·Sathish Kumar RamakrishnanCsilla Gergely
Nov 14, 2014·Journal of the Royal Society, Interface·Mailin MissonBo Jin
Jun 5, 2015·The Journal of Physical Chemistry. B·Gianvito GrassoAndrea Danani
Dec 14, 2016·ChemistryOpen·Hans-Christian RothSonja Berensmeier
Aug 3, 2017·Faraday Discussions·S P SchwamingerS Berensmeier

❮ Previous
Next ❯

Citations

Nov 28, 2020·Biotechnology Journal·Alexander A ZankerSonja Berensmeier
Apr 23, 2021·Biomaterials Research·Lucía Abarca-CabreraSonja Berensmeier
May 1, 2021·Pharmaceuticals·Chiara TurrinaSebastian P Schwaminger
Jul 25, 2021·International Journal of Molecular Sciences·Fabio VianelloMassimiliano Magro
Aug 29, 2019·ACS Omega·Sebastian P SchwamingerSonja Berensmeier
Oct 6, 2021·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Elena KorinaOleg Bol'shakov

❮ Previous
Next ❯

Methods Mentioned

BETA
peptide array

Software Mentioned

STOE WinXPOW
ProtParam Tool
ExPASy
ImageJ

Related Concepts

Related Feeds

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.

Related Papers

Faraday Discussions
S P SchwamingerS Berensmeier
Journal of Cellular Biochemistry
K B HellmanC F Fox
IEE Proceedings. Nanobiotechnology
Wolfgang Knoll, Wolfgang Fritzsche
Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics
P González-Mozuelos, M Olvera de la Cruz
Journal of Biomedical Materials Research. Part a
Wing-Hin LeeRamin Rohanizadeh
© 2022 Meta ULC. All rights reserved