PMID: 19139584Jan 14, 2009Paper

Binding properties of hydrophobic molecules to human serum albumin studied by fluorescence titration

Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry
Kô TakeharaShuto Yamada

Abstract

Two fluorescence modes were combined to analyze the binding properties of terminally substituted alkanes (C(n)X, X = COOH, OH, CHO, NH(2)) to human serum albumin (HSA). A competitive binding assay using an 8-anilino-1-naphthalenesulfonate (ANS) fluorescence probe provides information on all the hydrophobic binding sites in HSA. A binding assay using the intrinsic fluorescence of the tryptophan residue in HSA (Trp-HSA) provides information on the specific binding site close to the tryptophan residue. There are three fluorescence-active ANS binding sites in HSA, which can be classified into two types by their affinity for ANS. C(n)COOH bound to all three ANS binding sites including the Trp-HSA site, however, it did not quench the fluorescence of Trp-HSA. C(n)CHO bound only to the Trp-HSA site with quenching of the fluorescence of Trp-HSA. By comparing the binding affinities of HSA for C(n)OH and C(n)CHO, it was concluded that the C(n)OH binding site is different from the C(n)CHO binding site. C(n)NH(2) did not bind to any of the three ANS binding sites in HSA.

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Citations

May 18, 2013·Analytica Chimica Acta·Naoya KishikawaNaotaka Kuroda
Mar 27, 2013·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Priyankar SenRizwan Hasan Khan
Apr 12, 2011·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Shinya YamasakiKô Takehara
Jan 15, 2011·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Feng GeChaoyin Chen
Dec 2, 2011·Journal of Photochemistry and Photobiology. B, Biology·E AlarcónE A Lissi
Sep 17, 2013·Protein Science : a Publication of the Protein Society·Rajeev Yadav, Pratik Sen
Apr 1, 2016·Journal of Photochemistry and Photobiology. B, Biology·Yanqing WangJian Cao
Aug 13, 2015·Physical Chemistry Chemical Physics : PCCP·Shruti AryaSamrat Mukhopadhyay
Oct 2, 2009·Organic & Biomolecular Chemistry·Kwanghee Koh ParkAndrew D Hamilton
Apr 13, 2018·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Elnaz NakhaeiYoshiki Katayama
Oct 30, 2010·The Journal of Physical Chemistry. B·Matteo SalvalaglioCarlo Cavallotti
Mar 29, 2018·Chemical Research in Toxicology·Mary Jo Weiss-ErricoKevin E O'Shea
May 11, 2019·Journal of Medicinal Chemistry·Stephen BoultonGiuseppe Melacini

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