PMID: 8604322Feb 15, 1996Paper

Binding site analysis of c-Myb: screening of potential binding sites by using the mutation matrix derived from systematic binding affinity measurements

Nucleic Acids Research
Q L DengA Sarai

Abstract

The c-Myb oncoprotein is known to bind to multiple sites in the promoters of target genes. We have developed a protocol to screen the binding site of c-Myb by using the systematic binding data derived form measurements of binding affinity for oligonucleotide containing a known Myb-binding site and its complete single mutants. We first applied the method to predict the binding affinity for the known binding sites and compared with available experimental data. The predicated binding sites agree with many putative binding sites of known target promoters. However, there are some binding sites not predicated by the analysis. These sequences deviate from the consensus sequence derived from the binding analyses. In the light of the structure of Myb-DNA complex, these results indicate that different DNA-binding modes may be used by c-Myb to recognize different classes of binding sites. We also screened the sequence database for potential Myb-binding sites, and found sequences of several promoters that have not been identified experimentally but could be the target for c-Myb.

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Citations

Aug 16, 2003·Clinical Genetics·R C GreenJ S Green
Oct 2, 2002·Molecular Pathology : MP·C GaillardB Perbal
Jun 20, 2013·The Journal of Clinical Investigation·Henry F Frierson, Christopher A Moskaluk
Feb 12, 2013·Retrovirology·Mamneet Manghera, Renée N Douville
Mar 5, 2016·Biomarker Research·Wiltrud PrinzhornWolfgang Seifarth
Apr 8, 2015·Bone·V OralováM Buchtová
Mar 22, 2001·Blood Cells, Molecules & Diseases·C SicurellaR G Ramsay
Feb 24, 2015·Journal of Pharmaceutical and Biomedical Analysis·Xiwei ZhengDavid S Hage
Jul 27, 1999·The Journal of Biological Chemistry·K B AnderssonO S Gabrielsen
Mar 7, 1997·The Journal of Biological Chemistry·T L BrandtD J Kroll
Nov 9, 2002·The Journal of Biological Chemistry·Michele M LuchettiArmando Gabrielli
Oct 17, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Tyler M WeaverCatherine A Musselman
Aug 1, 2013·Oncology Reports·Divya Ramchandani, Georg F Weber

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