Binding sites of crustacean hyperglycemic hormone and its second messengers on gills and hindgut of the green shore crab, Carcinus maenas: a possible osmoregulatory role

General and Comparative Endocrinology
J Sook Chung, S G Webster

Abstract

To determine the possible involvement of crustacean hyperglycemic hormone (CHH) in osmoregulation in crustaceans, ligand binding and second messenger assays were performed on gills and hindgut preparations of the green shore crab Carcinus maenas, whilst midgut gland, previously known as one of the target tissues of CHH served as a control tissue. Classical receptor binding analyses using [(125)I]CHH by saturation and displacement experiments from membrane preparations from gills, hindgut, and midgut glands demonstrated that CHH binding characteristics involved one site, highly specific, saturable, and displaceable kinetics: (gills: K(D) 5.87 +/- 2.05 x 10(-10) and B(MAX) 6.50 +/- 1.15 x 10(-10), hindgut: K(D) 3.54 +/- 1.49 x 10(-10) and B(MAX) 2.31 +/- 0.44 x 10(-10), and midgut gland: K(D) 7.28 +/- 0.9 x 10(-10) and B(MAX) 3.28 +/- 0.25 x 10(-10)) all expressed as M/mg protein. No differences, in terms of displacement were observed between the two CHH isoforms (N-terminally blocked pGlu and unblocked Gln) variants. CHH binding sites appeared to be coupled to a second messenger system involving cGMP in all the tissues examined. Exposure of crabs to dilute seawater increased levels of cGMP, glucose in gills and circulating CHH l...Continue Reading

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Citations

May 21, 2011·Cell and Tissue Research·Andrew E Christie
Dec 15, 2015·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Natalia C PrymaczokDaniel A Medesani
Jan 25, 2012·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Su-Hua WuChi-Ying Lee
Dec 8, 2011·General and Comparative Endocrinology·Simon George WebsterHeinrich Dircksen
Dec 23, 2009·General and Comparative Endocrinology·J Sook ChungN Tsutsui
Jan 13, 2009·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Joseph A CoviDonald L Mykles
Jun 26, 2013·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Bo-Mi KimJae-Seong Lee
Apr 20, 2011·General and Comparative Endocrinology·Ernest S Chang, Donald L Mykles
Oct 2, 2012·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Sandra Fehsenfeld, Dirk Weihrauch
Feb 21, 2016·The Journal of Experimental Biology·Ran LiJin-Sheng Sun
Mar 15, 2018·Frontiers in Endocrinology·Éverton L VogtAnapaula S Vinagre
Mar 28, 2018·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Laura Camacho-JiménezElizabeth Ponce-Rivas
Oct 30, 2020·Frontiers in Endocrinology·Hsiang-Yin ChenChi-Ying Lee
Nov 15, 2014·The Journal of Experimental Biology·Moshe TomPiero Giulio Giulianini

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