PMID: 7519671Apr 1, 1994Paper

Binding sites of fluorescent probes on human serum albumin

The Journal of Pharmacy and Pharmacology
N MullerP Netter

Abstract

Human serum albumin is known to have two major and selective drug binding sites, termed sites I and II. The fluorescent probes, dansylamide and dansylsarcosine selectively interact with sites I and II, respectively. However, the binding site of the fluorescent probe dansylglycine on human serum albumin is not clear from the literature. This study investigated whether dansylglycine interacts tightly with site I or II. Spectrofluorimetric titrations (quenching and complex) and circular dichroism measurements were performed to determine the binding characteristics of dansylglycine to human serum albumin. Modification in probe fluorescence was described by fluorescence titrations to be a result of competitive displacement by ligands. The pattern of displacement of this probe by several ligands whose primary binding sites are exactly known, enabled the identification of its specific binding site. The fluorescence of dansylglycine is only extensively changed when ligands of site II are added, suggesting that it strongly interacts with the benzodiazepine/indole binding site on human serum albumin.

References

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Citations

Jan 1, 1996·Chirality·I MignotP Netter
Oct 24, 2002·Biochemical Pharmacology·Nicole A KratochwilPaul R Gerber
Aug 29, 2000·Anesthesia and Analgesia·M SueyasuR Oishi
Jun 26, 2002·Biological & Pharmaceutical Bulletin·Ulrich Kragh-HansenMasaki Otagiri
Oct 23, 2013·PloS One·Fernanda S Graciani, Valdecir F Ximenes
Jan 25, 2014·BMC Structural Biology·Annfrid SivertsenBjørn Olav Brandsdal
Oct 19, 2012·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Orsolya DömötörEva A Enyedy
Mar 5, 2013·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Maicon S PetrônioValdecir F Ximenes
Mar 5, 2004·Journal of Pharmaceutical Sciences·Wenying ZhongShuzi Tu
Jun 10, 2015·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Débora Naliati de Vasconcelos, Valdecir Farias Ximenes
Feb 23, 1996·Journal of Chromatography. B, Biomedical Applications·J OravcováW Lindner
Oct 31, 2008·Talanta·C André, Y C Guillaume
May 3, 2005·International Journal of Biological Macromolecules·Sebnem ErcelenAlexander P Demchenko
Apr 27, 2007·Journal of Chromatography. a·Ana TomasićVolker Magnus
Jan 8, 2014·Langmuir : the ACS Journal of Surfaces and Colloids·Tiffany P GustafsonKaren L Wooley
Nov 13, 2021·Journal of Biomolecular Structure & Dynamics·Amer M AlanaziAli Saber Abdelhameed

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