Binding sites of quinones in photosynthetic bacterial reaction centers investigated by light-induced FTIR difference spectroscopy: symmetry of the carbonyl interactions and close equivalence of the QB vibrations in Rhodobacter sphaeroides and Rhodopseudomonas viridis probed by isotope labeling

Biochemistry
Jacques BretonEliane Nabedryk

Abstract

The photoreduction of the secondary quinone acceptor QB in reaction centers (RCs) of the photosynthetic bacteria Rhodobacter sphaeroides and Rhodopseudomonas viridis has been investigated by light-induced FTIR difference spectroscopy of RCs reconstituted with several isotopically labeled ubiquinones. The labels used were 18O on both carbonyls and 13C either uniformly or selectively at the 1- or the 4-position, i.e., on either one of the two carbonyls. The QB-/QB spectra of RCs reconstituted with the isotopically labeled and unlabeled quinones as well as the double differences calculated from these spectra exhibit distinct isotopic shifts for a number of bands attributed to vibrations of QB and QB-. The vibrational modes of the quinone in the QB site are compared to those of ubiquinone in vitro, leading to band assignments for the C = O and C = C vibrations of the neutral QB and for the C***O and C***C of the semiquinone. The C = O frequency of each of the carbonyls of the unlabeled quinone is revealed at 1641 cm-1 for both species. This demonstrates symmetrical and weak hydrogen bonding of the two C = O groups to the protein at the QB site. In contrast, the C = C vibrations are not equivalent for selective labeling at C1 or at ...Continue Reading

Citations

Jun 11, 2009·Photosynthesis Research·Catherine Berthomieu, Rainer Hienerwadel
Jun 26, 2003·Bioorganic & Medicinal Chemistry Letters·Hiromi YabunakaHideto Miyoshi
Jan 20, 2004·European Journal of Biochemistry·André RemyKlaus Gerwert
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Oct 12, 2010·European Journal of Cell Biology·Delphine OnidasKlaus Gerwert
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Feb 4, 2019·Photosynthesis Research·Paul MathisAndré Verméglio
Feb 10, 2021·Advances in Colloid and Interface Science·Iman ZojajiJaber Taheri-Shakib
Mar 24, 2020·Chemical Reviews·Victor A Lorenz-Fonfria

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