Binding to complement factors and activation of the alternative pathway by Acanthamoeba

Immunobiology
Wilawan PumidonmingFranz Petry

Abstract

Acanthamoeba can cause severe ocular and cerebral diseases in healthy and immunocompromised individuals, respectively. Activation of complement appears to play an important role in host defence against infection. The exact mechanism, however, is still unclear. The aim of the present study was to investigate the effect of normal human serum (NHS) and normal mouse serum (NMS) on Acanthamoeba trophozoites, the binding of different complement factors to Acanthamoeba and the activation of the complement system. Moreover, we aimed to work out any possible differences between different strains of Acanthamoeba. A virulent T4 strain, a non-virulent T4 strain and a virulent T6 strain were included in the study. It was shown that NHS, but not NMS clearly has amoebicidal properties. After 5min of incubation with NHS, amoebae showed plasma membrane disruption and extrusion of intracellular components. Cells were completely destroyed within 60min of incubation in NHS but stayed intact after incubation in heat-inactivated serum. The binding of human C3 and C9 to amoebae was established by immunoblotting. Although incubation with mouse serum did not result in lysis of Acanthamoeba trophozoites an immunofluorescence assay (IFA) demonstrated a s...Continue Reading

References

Mar 1, 1991·Reviews of Infectious Diseases·A Ferrante
Nov 1, 1989·Infection and Immunity·T R KozelS M Levitz
Nov 1, 1985·Infection and Immunity·J Calderon, R D Schreiber
Aug 1, 1987·Infection and Immunity·T R KozelG S Pfrommer
Oct 1, 1987·Infection and Immunity·L Y Whiteman, F Marciano-Cabral
Sep 1, 1982·Journal of Neuropathology and Experimental Neurology·A J Martinez
Aug 1, 1980·Infection and Immunity·R T CursonsD Till
Jan 1, 1994·Transactions of the Royal Society of Tropical Medicine and Hygiene·L Flores-RomoA Martínez-Palomo
Nov 1, 1996·The Journal of Eukaryotic Microbiology·R J GastT J Byers
Jun 23, 1998·Survey of Ophthalmology·C D Illingworth, S D Cook
Mar 11, 1999·Experimental and Clinical Immunogenetics·M Botto
Dec 13, 2000·The Journal of Biological Chemistry·J HellwageS Meri
May 23, 2001·International Immunopharmacology·P KraiczyV Brade
Jul 1, 1952·Proceedings of the Society for Experimental Biology and Medicine·R B McGHEE
May 19, 2004·The Journal of Experimental Medicine·Lei ShiR Alan B Ezekowitz
Jan 24, 2006·Infection and Immunity·Angela E FritzingerFrancine Marciano-Cabral
May 8, 2007·Transactions of the Royal Society of Tropical Medicine and Hygiene·Meta RoestenbergAndré van der Ven
Feb 27, 2008·Seminars in Immunopathology·Nalini S BoraPuran S Bora
Dec 9, 2008·Experimental Parasitology·Maryam NiyyatiMostafa Rezaeian
Dec 17, 2008·Experimental Parasitology·Neil BrindleyNaveed Ahmed Khan
May 8, 2009·Cornea·F R S CarvalhoDenise de Freitas
Jul 8, 2009·Infection and Immunity·S Vicky TsoniGordon D Brown
Sep 11, 2009·Optometry and Vision Science : Official Publication of the American Academy of Optometry·Fiona StapletonKathy McClellan
Sep 19, 2009·Neuromolecular Medicine·Trent M WoodruffStephen M Taylor
Dec 17, 2009·Parasitology Research·Wilawan PumidonmingJulia Walochnik

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Citations

Mar 12, 2015·Molecular Biology and Evolution·Andrew E WebbMary J O'Connell
May 9, 2019·Parasitology Research·Jian-Ming HuangFu-Chin Huang

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