Bio-inspired oligovitronectin-grafted surface for enhanced self-renewal and long-term maintenance of human pluripotent stem cells under feeder-free conditions

Biomaterials
Hyun-Ji ParkSeung-Woo Cho

Abstract

Current protocols for human pluripotent stem cell (hPSC) expansion require feeder cells or matrices from animal sources that have been the major obstacle to obtain clinical grade hPSCs due to safety issues, difficulty in quality control, and high expense. Thus, feeder-free, chemically defined synthetic platforms have been developed, but are mostly confined to typical polystyrene culture plates. Here, we report a chemically defined, material-independent, bio-inspired surface coating allowing for feeder-free expansion and maintenance of self-renewal and pluripotency of hPSCs on various polymer substrates and devices. Polydopamine (pDA)-mediated immobilization of vitronectin (VN) peptides results in surface functionalization of VN-dimer/pDA conjugates. The engineered surfaces facilitate adhesion, proliferation, and colony formation of hPSCs via enhanced focal adhesion, cell-cell interaction, and biophysical signals, providing a chemically defined, xeno-free culture system for clonal expansion and long-term maintenance of hPSCs. This surface engineering enables the application of clinically-relevant hPSCs to a variety of biomedical systems such as tissue-engineering scaffolds and medical devices.

References

Nov 6, 1998·Science·J A ThomsonJ M Jones
Aug 26, 2004·The Journal of Biological Chemistry·Nicholas R D PalingMelanie J Welham
Feb 14, 2006·The International Journal of Biochemistry & Cell Biology·Barbara S MallonRonald D G McKay
Feb 9, 2007·Differentiation; Research in Biological Diversity·Jian LiHongkui Deng
May 29, 2007·Nature Biotechnology·Kiichi WatanabeYoshiki Sasai
Oct 20, 2007·Science·Haeshin LeePhillip B Messersmith
Aug 14, 2008·Nature Reviews. Molecular Cell Biology·Shin-ichi NishikawaConcepcion R Nierras
Oct 17, 2008·Molecular Cancer Research : MCR·Thomas R JohnsonHari K Koul
Feb 14, 2009·Journal of Chromatography. a·Xiaoyang ZhengWilliam S Hancock
Oct 6, 2009·Advanced Materials·Haeshin LeePhillip B Messersmith
Apr 22, 2010·Proceedings of the National Academy of Sciences of the United States of America·Yue XuSheng Ding
Jun 1, 2010·Nature Biotechnology·Luis G Villa-DiazGary D Smith
Jun 1, 2010·Nature Biotechnology·Sergey RodinKarl Tryggvason
Jan 18, 2011·Recent Patents on Nanotechnology·J J RoaM Segarra
May 24, 2011·Stem Cell Reviews and Reports·Toshio MikiMichael Kahn
Jul 2, 2011·Annals of Neurology·Jiho JangDong-Wook Kim
Dec 6, 2012·Nature Communications·Takamichi MiyazakiEihachiro Kawase
May 21, 2013·Cell·Masahito TachibanaShoukhrat Mitalipov
Oct 22, 2013·Nature Nanotechnology·Anthony ConwayDavid V Schaffer
May 1, 2012·International Journal of Stem Cells·Kisuk YangSeung-Woo Cho

❮ Previous
Next ❯

Citations

Feb 3, 2016·Angewandte Chemie·Chanoong LimDong Soo Hwang
Oct 3, 2015·Cellular and Molecular Life Sciences : CMLS·Siqin WuAnna Rising
Feb 14, 2017·Langmuir : the ACS Journal of Surfaces and Colloids·Mariagrazia IacominoMarco d'Ischia
Jul 20, 2017·ACS Applied Materials & Interfaces·Md Arifur RahimFrank Caruso
Jan 18, 2018·Scientific Reports·Jack W LambsheadAndrew L Laslett
Oct 24, 2018·Clinical Hemorheology and Microcirculation·Yan NieAndreas Lendlein
Mar 1, 2016·Current Stem Cell Reports·Jacob H JordahlJoerg Lahann
Jun 5, 2020·Tissue Engineering. Part a·Marianna Ferreira GonçalvesRegina Coeli Dos Santos Goldenberg
Feb 22, 2018·ACS Applied Materials & Interfaces·Ji Hyun RyuHaeshin Lee

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cadherins and Catenins

Cadherins (named for "calcium-dependent adhesion") are a type of cell adhesion molecule (CAM) that is important in the formation of adherens junctions to bind cells with each other. Catenins are a family of proteins found in complexes with cadherin cell adhesion molecules of animal cells: alpha-catenin can bind to β-catenin and can also bind actin. β-catenin binds the cytoplasmic domain of some cadherins. Discover the latest research on cadherins and catenins here.

AKT Pathway

This feed focuses on the AKT serine/threonine kinase, which is an important signaling pathway involved in processes such as glucose metabolism and cell survival.

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.