Abstract
Current protocols for human pluripotent stem cell (hPSC) expansion require feeder cells or matrices from animal sources that have been the major obstacle to obtain clinical grade hPSCs due to safety issues, difficulty in quality control, and high expense. Thus, feeder-free, chemically defined synthetic platforms have been developed, but are mostly confined to typical polystyrene culture plates. Here, we report a chemically defined, material-independent, bio-inspired surface coating allowing for feeder-free expansion and maintenance of self-renewal and pluripotency of hPSCs on various polymer substrates and devices. Polydopamine (pDA)-mediated immobilization of vitronectin (VN) peptides results in surface functionalization of VN-dimer/pDA conjugates. The engineered surfaces facilitate adhesion, proliferation, and colony formation of hPSCs via enhanced focal adhesion, cell-cell interaction, and biophysical signals, providing a chemically defined, xeno-free culture system for clonal expansion and long-term maintenance of hPSCs. This surface engineering enables the application of clinically-relevant hPSCs to a variety of biomedical systems such as tissue-engineering scaffolds and medical devices.
References
Nov 6, 1998·Science·J A ThomsonJ M Jones
Aug 6, 2002·Nature Biotechnology·Mark RichardsAriff Bongso
Aug 26, 2004·The Journal of Biological Chemistry·Nicholas R D PalingMelanie J Welham
Feb 14, 2006·The International Journal of Biochemistry & Cell Biology·Barbara S MallonRonald D G McKay
Feb 9, 2007·Differentiation; Research in Biological Diversity·Jian LiHongkui Deng
May 29, 2007·Nature Biotechnology·Kiichi WatanabeYoshiki Sasai
Oct 20, 2007·Science·Haeshin LeePhillip B Messersmith
Nov 24, 2007·Cell·Kazutoshi TakahashiShinya Yamanaka
Jul 5, 2008·Stem Cells·Stefan R BraamChristine L Mummery
Aug 14, 2008·Nature Reviews. Molecular Cell Biology·Shin-ichi NishikawaConcepcion R Nierras
Oct 17, 2008·Molecular Cancer Research : MCR·Thomas R JohnsonHari K Koul
Feb 14, 2009·Journal of Chromatography. a·Xiaoyang ZhengWilliam S Hancock
Oct 6, 2009·Advanced Materials·Haeshin LeePhillip B Messersmith
Apr 22, 2010·Proceedings of the National Academy of Sciences of the United States of America·Yue XuSheng Ding
Jun 1, 2010·Nature Biotechnology·Zara MelkoumianRalph Brandenberger
Jun 1, 2010·Nature Biotechnology·Luis G Villa-DiazGary D Smith
Jun 1, 2010·Nature Biotechnology·Sergey RodinKarl Tryggvason
Jun 4, 2010·Stem Cells·Taotao ChenGang Pei
Aug 24, 2010·Nature Materials·Ying MeiDaniel G Anderson
Sep 8, 2010·Biomaterials·David A BrafmanShu Chien
Dec 24, 2010·PloS One·Farhan ChowdhuryTetsuya S Tanaka
Jan 18, 2011·Recent Patents on Nanotechnology·J J RoaM Segarra
Feb 25, 2011·Chemical Reviews·Akon HiguchiAkihiro Umezawa
May 24, 2011·Stem Cell Reviews and Reports·Toshio MikiMichael Kahn
Jul 2, 2011·Annals of Neurology·Jiho JangDong-Wook Kim
Jul 26, 2011·Journal of Signal Transduction·Pierre H Vachon
May 25, 2012·Lab on a Chip·Sewoon HanSeung-Woo Cho
Jul 20, 2012·Biomaterials·Kisuk YangSeung-Woo Cho
Dec 6, 2012·Nature Communications·Takamichi MiyazakiEihachiro Kawase
May 21, 2013·Cell·Masahito TachibanaShoukhrat Mitalipov
Aug 15, 2013·Biomacromolecules·Eunkyung KoSeung-Woo Cho
Oct 22, 2013·Nature Nanotechnology·Anthony ConwayDavid V Schaffer
Nov 1, 2013·Biomaterials·Tandis VazinDavid V Schaffer
Dec 3, 2013·Biomaterials·Hee Seok YangDeok-Ho Kim
May 1, 2012·International Journal of Stem Cells·Kisuk YangSeung-Woo Cho
Citations
Feb 22, 2016·Biomaterials·Ping ZhouShicheng Wei
Feb 3, 2016·Angewandte Chemie·Chanoong LimDong Soo Hwang
Dec 15, 2015·Scientific Reports·Akon HiguchiAkihiro Umezawa
Nov 1, 2015·Biomaterials·I-Chia PengAkon Higuchi
Oct 3, 2015·Cellular and Molecular Life Sciences : CMLS·Siqin WuAnna Rising
Apr 14, 2016·Nanoscale·Jin KimSeung-Woo Cho
Jun 21, 2016·ACS Applied Materials & Interfaces·Kisuk YangSeung-Woo Cho
Sep 11, 2016·Stem Cell Research & Therapy·Michele M NavaManuela T Raimondi
Oct 6, 2016·Nanoscale·Meiying LiuYen Wei
Oct 8, 2016·Small·Jisoo ShinSeung-Woo Cho
Jan 10, 2017·Biomacromolecules·Yi DengShicheng Wei
Feb 14, 2017·Langmuir : the ACS Journal of Surfaces and Colloids·Mariagrazia IacominoMarco d'Ischia
Mar 24, 2017·Scientific Reports·Yen-Ming ChenAkihiro Umezawa
Jul 1, 2017·ACS Applied Materials & Interfaces·Michinari KohriKeiki Kishikawa
Jul 20, 2017·ACS Applied Materials & Interfaces·Md Arifur RahimFrank Caruso
Aug 5, 2017·Materials·Davide RicciRoberto Osellame
Jan 18, 2018·Scientific Reports·Jack W LambsheadAndrew L Laslett
Oct 24, 2018·Clinical Hemorheology and Microcirculation·Yan NieAndreas Lendlein
Aug 15, 2019·Biomaterials Science·Li-Hua ChenAkihiro Umezawa
Sep 13, 2017·ChemSusChem·Konstantin S RodyginValentine P Ananikov
Nov 8, 2018·International Journal of Molecular Sciences·Nan HaiSha Jin
Mar 1, 2016·Current Stem Cell Reports·Jacob H JordahlJoerg Lahann
Jun 5, 2020·Tissue Engineering. Part a·Marianna Ferreira GonçalvesRegina Coeli Dos Santos Goldenberg
Aug 6, 2019·Advanced Science·Jinping ZhaoJiuhong Kang
Jan 26, 2020·Stem Cell Research·Ahmed Abdal DayemSsang-Goo Cho
Mar 1, 2019·Acta Biomaterialia·Huan LiuChangsheng Liu
Feb 22, 2018·ACS Applied Materials & Interfaces·Ji Hyun RyuHaeshin Lee
Aug 26, 2021·Advanced Materials·André RulandUwe Freudenberg