Bio-sensing of cadmium(II) ions using Staphylococcus aureus.

Sensors
Jiri SochorRene Kizek

Abstract

Cadmium, as a hazardous pollutant commonly present in the living environment, represents an important risk to human health due to its undesirable effects (oxidative stress, changes in activities of many enzymes, interactions with biomolecules including DNA and RNA) and consequent potential risk, making its detection very important. New and unique technological and biotechnological approaches for solving this problems are intensely sought. In this study, we used the commonly occurring potential pathogenic microorganism Staphylococcus aureus for the determination of markers which could be used for sensing of cadmium(II) ions. We were focused on monitoring the effects of different cadmium(II) ion concentrations (0, 1.25, 2.5, 5, 10, 15, 25 and 50 μg mL(-1)) on the growth and energetic metabolism of Staphylococcus aureus. Highly significant changes have been detected in the metabolism of thiol compounds-specifically the protein metallothionein (0.79-26.82 mmol/mg of protein), the enzyme glutathione S-transferase (190-5,827 μmol/min/mg of protein), and sulfhydryl groups (9.6-274.3 μmol cysteine/mg of protein). The ratio of reduced and oxidized glutathione indicated marked oxidative stress. In addition, dramatic changes in urease act...Continue Reading

References

Mar 1, 1992·Microbiological Reviews·S Silver, M Walderhaug
Feb 1, 1989·Trends in Biochemical Sciences·S SilverT K Misra
May 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·G NuciforaS Silver
Apr 1, 1968·Journal of Bacteriology·R P Novick, C Roth
Feb 7, 1997·Journal of Chromatography. B, Biomedical Sciences and Applications·R Causon
Jan 7, 1998·Trends in Biotechnology·S RamanathanS Daunert
Jul 28, 1999·Applied Microbiology and Biotechnology·D H Nies
Mar 7, 2000·Ecotoxicology and Environmental Safety·M R BruinsF W Oehme
Feb 13, 2002·Biosensors & Bioelectronics·V Venugopal
Nov 27, 2002·Die Naturwissenschaften·Fengxiang X HanGlover E Triplett
Apr 4, 2003·Analytical Biochemistry·Israel BiranDavid R Walt
Apr 23, 2003·Biosensors & Bioelectronics·R BhatiaD M Rawson
Jun 28, 2003·FEMS Microbiology Reviews·Dietrich H Nies
Aug 14, 2003·Ecotoxicology and Environmental Safety·Christopher Rensing, Raina M Maier
May 1, 1959·Archives of Biochemistry and Biophysics·G L ELLMAN
Nov 19, 2003·Biotechnology Advances·Yossi PaitanEliora Z Ron
Aug 28, 2007·International Journal of Occupational and Environmental Health·James HuffPeter F Infante
Sep 1, 2007·Analytica Chimica Acta·Yu LeiAshok Mulchandani
Nov 23, 2007·Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie·Samir HaouemRachid Sakly
Dec 12, 2007·Journal of Basic Microbiology·Götz Haferburg, Erika Kothe
Aug 9, 2008·Journal of Environmental Monitoring : JEM·Ademola O OlaniranBalakrishna Pillay
Jan 10, 2009·Biosensors & Bioelectronics·Hadar Ben-YoavYosi Shacham-Diamand
Aug 20, 2009·Current Protein & Peptide Science·Tomas EckschlagerRene Kizek

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Citations

Oct 24, 2012·Environmental Science and Pollution Research International·S M El-Sonbaty, D E El-Hadedy
Apr 23, 2013·Research in Microbiology·Hideki KinoshitaKazuhiko Kimura
Dec 18, 2013·Toxins·Pratik BanerjeeBalabhaskar Prabhakarpandian
Jan 15, 2014·Fish Physiology and Biochemistry·Bidhan C DeA P Sharma

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Methods Mentioned

BETA
biosensor
biosensors
biosensing

Software Mentioned

WinASPECT
Matlab
GPES
BS
MEDUSA
AnalyticJena
EcoChemie
Mindray
Ascent

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