Bioactive cell-derived matrices combined with polymer mesh scaffold for osteogenesis and bone healing

Biomaterials
In Gul KimKwideok Park

Abstract

Successful bone tissue engineering generally requires an osteoconductive scaffold that consists of extracellular matrix (ECM) to mimic the natural environment. In this study, we developed a PLGA/PLA-based mesh scaffold coated with cell-derived extracellular matrix (CDM) for the delivery of bone morphogenic protein (BMP-2), and assessed the capacity of this system to provide an osteogenic microenvironment. Decellularized ECM from human lung fibroblasts (hFDM) was coated onto the surface of the polymer mesh scaffolds, upon which heparin was then conjugated onto hFDM via EDC chemistry. BMP-2 was subsequently immobilized onto the mesh scaffolds via heparin, and released at a controlled rate. Human placenta-derived mesenchymal stem cells (hPMSCs) were cultured in such scaffolds and subjected to osteogenic differentiation for 28 days in vitro. The results showed that alkaline phosphatase (ALP) activity, mineralization, and osteogenic marker expression were significantly improved with hPMSCs cultured in the hFDM-coated mesh scaffolds compared to the control and fibronectin-coated ones. In addition, a mouse ectopic and rat calvarial bone defect model was used to examine the feasibility of current platform to induce osteogenesis as well...Continue Reading

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Citations

Dec 29, 2015·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·L CioccaR Scotti
Sep 1, 2016·ACS Applied Materials & Interfaces·Zhaozhao DingDavid L Kaplan
May 5, 2017·ACS Applied Materials & Interfaces·Zhaozhao DingDavid L Kaplan
Nov 25, 2017·Advanced Healthcare Materials·Hwan D KimNathaniel S Hwang
Jul 16, 2019·Advanced Healthcare Materials·In Gul KimKwideok Park
Jan 10, 2020·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·João C SilvaRobert J Linhardt
Oct 17, 2019·Journal of Biomedical Materials Research. Part a·Radoslaw JunkaXiaojun Yu
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Apr 6, 2020·Biomaterials Science·Liangliang YangPatrick van Rijn
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