Bioactive compounds from Mexican lime ( Citrus aurantifolia ) juice induce apoptosis in human pancreatic cells

Journal of Agricultural and Food Chemistry
Jaiprakash R PatilBhimanagouda S Patil

Abstract

Lime (Citrus aurantifolia Swingle) is one of the major citrus fruits and widely consumed, but there is limited evidence about its health-promoting properties. Hence, an investigation was conducted to understand the chemopreventive effects of lime juice on pancreatic cancer cells and the possible mechanism for induction of apoptosis using Panc-28 cells. Freeze-dried lime juice was extracted with different solvents, such as chloroform, acetone, MeOH, and MeOH/water (8:2). The chloroform extract showed the highest (85.4 and 90%) radical-scavenging activity by 1,1-diphenyl-2-picryl hydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) methods at 624 microg/mL, whereas the MeOH/water extract showed the lowest (<20%) activity. The active components were identified by high-performance liquid chromatography (HPLC) using a C-18 column as rutin, neohesperidin, hesperidin, and hesperitin. Furthermore, the limonoids identified are limonexic acid, isolimonexic acid, and limonin. All of the extracts of lime juice inhibited Panc-28 cancer cell growth. The MeOH extract exhibited the maximum activity, with an IC50 value of 81.20 microg/mL after 72 h. The inhibition of Panc-28 cells was in the range of 73-89%, at 100 m...Continue Reading

References

Jul 1, 1995·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·B HalliwellO I Aruoma
Jul 1, 1994·International Journal of Cancer. Journal International Du Cancer·A ShibataB E Henderson
Aug 28, 1998·Science·D R Green, J C Reed
Dec 20, 1999·Journal of Agricultural and Food Chemistry·S KawaiiM Yano
Dec 20, 1999·Journal of Agricultural and Food Chemistry·P RapisardaA Saija
Nov 21, 2001·Journal of Agricultural and Food Chemistry·J A VinsonP Bose
Jan 5, 2002·Journal of Agricultural and Food Chemistry·R P SinghG K Jayaprakasha
Sep 13, 2002·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Loïc Le Marchand
Dec 18, 2002·Annals of the New York Academy of Sciences·Alok C Bharti, Bharat B Aggarwal
Jan 9, 2003·Journal of Agricultural and Food Chemistry·Wei Zheng, Shiow Y Wang
Dec 10, 2003·Oncogene·Jordan S Fridman, Scott W Lowe
Mar 31, 2004·Lancet·Donghui LiJames L Abbruzzese
Dec 29, 2004·Journal of Pharmaceutical and Biomedical Analysis·M L CalabròR Ficarra
Mar 30, 2005·The Journal of Nutrition·Shibu M PouloseBhimanagouda S Patil
Aug 23, 2005·The Journal of Nutritional Biochemistry·Kun GaoDavid Heber
Jan 3, 2006·Carcinogenesis·Jairam VanamalaNancy D Turner
Jan 24, 2006·Bioscience, Biotechnology, and Biochemistry·Yoichi NogataHideaki Ohta
Jun 3, 2006·Journal of Cellular Physiology·Luciana E Giono, James J Manfredi
Oct 19, 2006·Life Sciences·Igor N SergeevSlavik Dushenkov
Jun 5, 2007·Zeitschrift Für Naturforschung. C, a Journal of Biosciences·Kranthi K MandadiBhimanagouda S Patil
Sep 28, 2007·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·H J ParkJ-H Chung
Feb 22, 2008·CA: a Cancer Journal for Clinicians·Ahmedin JemalMichael J Thun
May 21, 2008·Bioorganic & Medicinal Chemistry·G K JayaprakashaBhimanagouda S Patil
Jul 22, 2008·Biochemical Pharmacology·Isabel Marzo, Javier Naval

❮ Previous
Next ❯

Citations

Jun 29, 2011·Journal of Toxicology and Environmental Health. Part a·Zahira Fernández-BedmarJoaquín Pérez-Guisado
Jan 30, 2013·The Journal of Alternative and Complementary Medicine : Research on Paradigm, Practice, and Policy·Samuel Ademola AdegokeOyeku Akibu Oyelami
Jul 8, 2011·Digestive Diseases·Claudia NitscheMarkus M Lerch
Aug 16, 2014·Pharmacognosy Reviews·Katrin Sak
Dec 24, 2011·Evidence-based Complementary and Alternative Medicine : ECAM·Do-Hoon LeeGon-Sup Kim
Dec 2, 2015·Oncology Letters·Octavian Tudorel OlaruAristidis M Tsatsakis
Nov 17, 2012·European Journal of Medicinal Chemistry·Yong YangMauro Ferrari
Dec 7, 2010·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·I Alvarez-GonzalezJ J Espinosa-Aguirre
Aug 4, 2015·Journal of Clinical Biochemistry and Nutrition·Satomi ShimizuMichihiro Mutoh
Jan 6, 2011·Molecular Nutrition & Food Research·Jodee Johnson, Elvira Gonzalez de Mejia
Oct 9, 2012·Cell Biochemistry and Function·Yong YangMauro Ferrari
Mar 18, 2011·Phytotherapy Research : PTR·S A AdegokeL A Adeyemi
Jan 28, 2010·International Journal of Cancer. Journal International Du Cancer·Wen-Qing LiIchiro Tsuji
Mar 4, 2011·American Journal of Surgery·Barbara ZarebczanHerbert Chen
May 31, 2014·The International Journal of Biochemistry & Cell Biology·Lin Li, Po Sing Leung
Jun 11, 2014·Critical Reviews in Food Science and Nutrition·S RajarathnamS Rajarathnam
Apr 11, 2015·Cellular Oncology (Dordrecht)·Zeynep Birsu CincinBedia Cakmakoglu
Jun 26, 2012·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Jinhee KimBhimanagouda S Patil
Sep 20, 2011·European Journal of Pharmacology·Jinhee KimBhimanagouda S Patil
Nov 6, 2013·Critical Reviews in Food Science and Nutrition·Rosa TundisFrancesco Menichini
Nov 16, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Roberta GualdaniSolomon Habtemariam
Jul 6, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yue WangChongde Sun
Jan 16, 2016·Preventive Nutrition and Food Science·Gi Dae Kim
Dec 21, 2010·Journal of Separation Science·Guddadarangavvanahally K JayaprakashaBhimanagouda S Patil
Feb 13, 2010·Endocrine Practice : Official Journal of the American College of Endocrinology and the American Association of Clinical Endocrinologists·Adeola A SalawuAbayomi O Okanlawon
Oct 17, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Shunming FanLingying Yu

❮ Previous
Next ❯

Related Concepts

Related Feeds

BCL-2 Family Proteins

BLC-2 family proteins are a group that share the same homologous BH domain. They play many different roles including pro-survival signals, mitochondria-mediated apoptosis and removal or damaged cells. They are often regulated by phosphorylation, affecting their catalytic activity. Here is the latest research on BCL-2 family proteins.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis