Bioactive endophytes warrant intensified exploration and conservation.

PloS One
Stephen A SmithScott A Strobel

Abstract

A key argument in favor of conserving biodiversity is that as yet undiscovered biodiversity will yield products of great use to humans. However, the link between undiscovered biodiversity and useful products is largely conjectural. Here we provide direct evidence from bioassays of endophytes isolated from tropical plants and bioinformatic analyses that novel biology will indeed yield novel chemistry of potential value. We isolated and cultured 135 endophytic fungi and bacteria from plants collected in Peru. nrDNAs were compared to samples deposited in GenBank to ascertain the genetic novelty of cultured specimens. Ten endophytes were found to be as much as 15-30% different than any sequence in GenBank. Phylogenetic trees, using the most similar sequences in GenBank, were constructed for each endophyte to measure phylogenetic distance. Assays were also conducted on each cultured endophyte to record bioactivity, of which 65 were found to be bioactive. The novelty of our contribution is that we have combined bioinformatic analyses that document the diversity found in environmental samples with culturing and bioassays. These results highlight the hidden hyperdiversity of endophytic fungi and the urgent need to explore and conserve ...Continue Reading

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Citations

Jul 19, 2011·Applied and Environmental Microbiology·Jonathan R RussellScott A Strobel
Jun 2, 2011·Natural Product Reports·Souvik Kusari, Michael Spiteller
Jan 26, 2016·Microbiological Research·Gustavo SantoyoBernard R Glick
Sep 5, 2014·Conservation Biology : the Journal of the Society for Conservation Biology·Jacob Heilmann-ClausenPanu Halme
Oct 3, 2009·Microbiology·Angela M MitchellJoe Sears
Feb 6, 2019·Applied Microbiology and Biotechnology·Sidra RahmanZabta Khan Shinwari
Feb 28, 2019·International Microbiology : the Official Journal of the Spanish Society for Microbiology·Tehmina KianiSumaira Farrakh
Nov 22, 2017·Current Microbiology·Ana M L CorreiaAndre Rodrigues
Sep 21, 2019·Current Protocols in Pharmacology·John A Beutler
Aug 6, 2013·Tetrahedron Letters·Ezra G BarabanJo Handelsman
Apr 23, 2011·Journal of Natural Products·Camila Martins de OliveiraAngela Regina Araujo

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Methods Mentioned

BETA
PCR

Software Mentioned

Muscle
MrBayes
Phyutility

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