Bioactive protein-based nanofibers interact with intestinal biological components resulting in transepithelial permeation of a therapeutic protein

International Journal of Pharmaceutics
Karen StephansenHanne Mørck Nielsen

Abstract

Proteins originating from natural sources may constitute a novel type of material for use in drug delivery. However, thorough understanding of the behavior and effects of such a material when processed into a matrix together with a drug is crucial prior to further development into a drug product. In the present study the potential of using bioactive electrospun fish sarcoplasmic proteins (FSP) as a carrier matrix for small therapeutic proteins was demonstrated in relation to the interactions with biological components of the intestinal tract. The inherent structural and chemical properties of FSP as a biomaterial facilitated interactions with cells and enzymes found in the gastrointestinal tract and displayed excellent biocompatibility. More specifically, insulin was efficiently encapsulated into FSP fibers maintaining its conformation, and subsequent controlled release was obtained in simulated intestinal fluid. The encapsulation of insulin into FSP fibers provided protection against chymotrypsin degradation, and resulted in an increase in insulin transport to around 12% without compromising the cellular viability. This increased transport was driven by interactions upon contact between the nanofibers and the Caco-2 cell monol...Continue Reading

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Citations

Feb 14, 2016·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Saad M AlshehriTansir Ahamad
Apr 6, 2017·Macromolecular Bioscience·Alan R MackieLuciano Marcelloni
Feb 6, 2018·Annual Review of Food Science and Technology·Charlotte JacobsenIoannis S Chronakis
Mar 12, 2020·Frontiers in Bioengineering and Biotechnology·Spase Stojanov, Aleš Berlec
Dec 20, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ana C MendesIoannis S Chronakis
Feb 4, 2021·Drug Delivery and Translational Research·Valentina AndrettoGiovanna Lollo
Nov 1, 2020·Journal of Controlled Release : Official Journal of the Controlled Release Society·Anabela MoreiraGareth R Williams
Sep 28, 2017·Journal of Agricultural and Food Chemistry·Peng WenHong Wu
Oct 14, 2021·Journal of Nanobiotechnology·Hassan MalekiFarshad Sharifi

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