Bioactivity guided isolation of antipsychotic constituents from the leaves of Rauwolfia tetraphylla L

Fitoterapia
Shikha GuptaSantosh K Srivastava

Abstract

This study was undertaken to ascertain the antipsychotic properties of Rauwolfia tetraphylla L. leaves and to isolate and characterize the antipsychotic constituents. Among the MeOH extract and some alkaloidal fractions at different pHs, the alkaloidal CHCl(3) fraction at pH-9 (2C) showed the highest antipsychotic activity against dopaminergic (DA-D(2)) and serotonergic (5-HT(2A)) receptors in-vitro and amphetamine induced hyperactive mouse model in-vivo. The activity guided isolation of CHCl(3) fraction (2C) afforded six indole alkaloids: 10-methoxytetrahydroalstonine (1), isoreserpiline (2), an isomeric mixture of 11-demethoxyreserpiline (3) and 10-demethoxyreserpiline (4), α-yohimbine (5) and reserpiline (6). Given orally, alkaloids 3-6 showed significant antipsychotic activity in a dose dependent manner. None of the extract, alkaloidal fractions or alkaloids showed any extra pyramidal symptoms at the tested doses. It was also observed that MeOH extract was behaving similar to other clinically used novel atypical antipsychotics in having 5-HT(2A) occupancy greater than the DA-D(2) receptor at the tested doses. Further toxicity and safety evaluation studies of MeOH extracts of R. tetraphylla leaves at different doses (10, 100...Continue Reading

References

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Citations

Jul 2, 2015·Chemical Biology & Drug Design·Gaurav Raj DwivediSantosh K Srivastava
Dec 3, 2014·Parasitology International·Pone Kamdem BonifaceAnirban Pal
Jan 28, 2014·Current Therapeutic Research, Clinical and Experimental·Jun YuQi Chen
Dec 14, 2017·Evidence-based Complementary and Alternative Medicine : ECAM·Angel Josabad Alonso-CastroJuan Jose Maldonado-Miranda
Jun 20, 2020·Archiv der Pharmazie·Yang LiuGuishun Piao
Oct 13, 2020·Chemistry & Biodiversity·Guanqun ZhanZengjun Guo

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