Bioactivity of periodontal ligament stem cells on sodium titanate coated with graphene oxide

Scientific Reports
Qi ZhouShaohua Ge

Abstract

As a biocompatible and low cytotoxic nanomaterial, graphene oxide (GO) has captured tremendous interests in tissue engineering. However, little is known about the behavior of dental stem cells on GO. This study was to evaluate the bioactivity of human periodontal ligament stem cells (PDLSCs) on GO coated titanium (GO-Ti) substrate in vitro as compared to sodium titanate (Na-Ti) substrate. By scanning electron microscope (SEM), confocal laser scanning microscope (CLSM), methylthiazol tetrazolium (MTT) assay, alkaline phosphatase (ALP) activity, quantitative real-time polymerase chain reaction (qRT-PCR) and western blot analysis, we investigated the attachment, morphology, proliferation and osteogenic differentiation of PDLSCs on these two substrates. When seeded on GO-Ti substrate, PDLSCs exhibited significantly higher proliferation rate, ALP activity and up-regulated gene expression level of osteogenesis-related markers of collagen type I (COL-I), ALP, bone sialoprotein (BSP), runt related transcription factor 2 (Runx2) and osteocalcin (OCN) compared with those on Na-Ti substrate. Moreover, GO promoted the protein expression of BSP, Runx2 and OCN. These findings suggest that the combination of GO and PDLSCs provides a promising...Continue Reading

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Citations

May 13, 2017·Dental Materials : Official Publication of the Academy of Dental Materials·Han XieVinicius Rosa
Apr 4, 2018·Journal of Biomedical Materials Research. Part a·Dorsa MohammadrezaeiLobat Tayebi
Jan 25, 2020·Advanced Healthcare Materials·Zhili PengRoger M Leblanc
May 30, 2020·International Journal of Molecular Sciences·Joo-Young ParkJeong-Ho Yun
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Jan 18, 2020·Materials·Goker FundaMassimo Del Fabbro
Sep 11, 2020·Nanomaterials·Victoria Bonilla-RepresaM Olga Guerrero-Pérez

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Methods Mentioned

BETA
atomic force microscopy
AFM
PCR
flow cytometry
protein assay

Software Mentioned

Image J

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