PMID: 8956340Nov 1, 1996Paper

Bioadhesion of lectin-latex conjugates to rat intestinal mucosa

Pharmaceutical Research
J M IracheG Ponchel

Abstract

The specific interactions between three lectin-latex conjugates and different structures of rat intestinal mucosa have been studied ex vivo. These systems were prepared by covalent coupling of different ligands, i.e., tomato lectin (TL), asparagus pea lectin (AL), mycoplasma gallisepticum lectin (ML), and bovine serum albumin (BSA) as control, to poly(styrene) latexes. Using mucosa samples without Peyer's patches (PP), the extent of interaction of all three lectin-latex conjugates with the mucosa decreased from duodenum to ileum, probably due to progressive diminution of the mucin concentration along the gastrointestinal tract. The following order of interaction of the conjugates with the mucus gel layer was observed: TL > ML = AL (p < 0.05). For each lectin, these results corresponded well to the concentration of its specific sugar in the mucus. Using intestinal samples with PP, an important increase of interaction of the conjugates with the mucosa was found for ML (about 25%) and AL (about 50%), whereas the interaction of TL decreased about 25%. Photomicrographs with fluorescent latexes have confirmed the specificity of the ML- and AL-latex conjugates for the PP region and of the TL-latex conjugates for the mucus gel.

Citations

Sep 3, 2004·Current Gastroenterology Reports·David R Friend
Nov 2, 2004·Chemical & Pharmaceutical Bulletin·Jo VandervoortAnnick Ludwig
Nov 22, 2005·Expert Opinion on Drug Delivery·Krassimira YonchevaJuan M Irache
Nov 29, 2007·Journal of Drug Targeting·Christine VauthierGilles Ponchel
Dec 30, 2009·Pharmaceutical Development and Technology·Krassimira YonchevaAnnick Ludwig
Nov 2, 2017·Expert Review of Gastroenterology & Hepatology·Darren Michael MossAndrew Owen
Nov 17, 2007·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Juan M IrachePau Arbos
Jul 3, 2010·Expert Review of Clinical Immunology·Arik DahanEllen M Zimmermann
Apr 25, 2017·Critical Reviews in Biotechnology·Hedieh MalekzadMichael R Hamblin
Mar 18, 2000·Journal of Drug Targeting·J F Woodley
Sep 1, 2000·Advanced Drug Delivery Reviews·M A ClarkM A Jepson
Mar 10, 2000·Journal of Controlled Release : Official Journal of the Controlled Release Society·A Bernkop-SchnürchR G Biebel
Oct 18, 2000·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·M A ArangoaJ M Irache
Feb 19, 2004·Advanced Drug Delivery Reviews·Christiane BiesJohn F Woodley
Jan 13, 2006·Journal of Controlled Release : Official Journal of the Controlled Release Society·P L BardonnetF Falson
Nov 14, 2006·Journal of Controlled Release : Official Journal of the Controlled Release Society·Yashu YinHaiyang Hu
Oct 20, 2006·Journal of Controlled Release : Official Journal of the Controlled Release Society·Anne des RieuxVéronique Préat
Aug 31, 2007·Journal of Controlled Release : Official Journal of the Controlled Release Society·YaShu YinHaiYang Hu
Jan 10, 2009·Advanced Drug Delivery Reviews·Samuel K LaiJustin Hanes

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.