Bioaffinity Mass Spectrometry Screening

Journal of Biomolecular Screening
Ben YangRonald J Quinn

Abstract

Electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry (ESI-FTICR-MS or ESI-FTMS) was used to screen 192 natural product extracts and a 659-member natural product-based fragment library for bindings to a potential malaria drug target, Plasmodium falciparum Rab11a (PfRab11a, PF13_0119). One natural product extract and 11 fragments showed binding activity. A new natural product, arborside E, was identified from the active extract of Psydrax montigena as a weak binder. Its binding activity and inhibitory activity against PfRab11a were confirmed by ESI-FTMS titration experiments and an orthogonal enzyme assay.

References

Jul 11, 1998·Analytical Biochemistry·M K GreenB S Larsen
Mar 27, 2003·Gene·Emmanuel QuevillonGordon Langsley
Apr 29, 2006·Molecular and Biochemical Parasitology·Christopher MehlinWim G J Hol
Mar 1, 1985·Biophysical Chemistry·A Fernández, O Sinanoglu
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Mar 20, 2008·Journal of Biomolecular Screening·Hoan VuRonald J Quinn
Sep 13, 2011·Cell·Louis H Miller, Xinzhuan Su
Dec 30, 2011·Journal of Natural Products·David CampRonald J Quinn

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Citations

Jan 17, 2016·Journal of Biomolecular Screening·Jonathan Wingfield, Ian D Wilson
Jun 6, 2017·SLAS Discovery·Erica C VanderPortenYichin Liu
Oct 8, 2017·Essays in Biochemistry·Daniel Shiu-Hin ChanChris Abell
May 28, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ali R ElnaasMiaomiao Liu
Jan 29, 2021·Scientific Reports·Miaomiao LiuRonald J Quinn
Aug 28, 2021·Chemical Reviews·Jack L BennettWilliam A Donald
Dec 11, 2021·Journal of the American Chemical Society·Giang T H NguyenWilliam A Donald

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Methods Mentioned

BETA
GTPases
NMR
GTPase

Software Mentioned

Windows
GraphPad Prism
Xmass
Prism

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