Bioanalysis of tobramycin for therapeutic drug monitoring by solid-phase extraction and capillary zone electrophoresis

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Humphrey Azambeh FongeJos Hoogmartens

Abstract

A method based on solid-phase extraction (SPE) and capillary zone electrophoresis (CZE) for the analysis of tobramycin in human serum is presented. An off-line SPE employing a carboxypropyl bonded phase (CBA) cartridge was used for the extraction of tobramycin from human serum. Adsorbed tobramycin was eluted from the CBA cartridge using a mixture of NH(3) (25%, w/v)-methanol (30:70, v/v). After evaporation, the analyte was reconstituted and derivatized with o-phthaldialdehyde (OPA)/3-mercaptopropionic acid (MPA). The resulting tobramycin-OPA/MPA derivative was purified, and then identified by mass spectrometry. The tobramycin-OPA/MPA derivative was then analysed by CZE with a background electrolyte (BGE) comprising of 30 mM sodium tetraborate pH 10.0-acetonitrile (ACN) (80:20, v/v) with ultraviolet detection at 230 nm. A linear response was observed in the range of 0.3-30 microg/ml with r(2) = 0.992. The sensitivity of the method was determined by its limit of quantitation (LOQ) and limit of detection (LOD) of 0.3 microg/ml and 0.1 microg/ml, respectively. SPE recovery ranged from 68 to 79% at the trough levels to 98% at the peak levels found in serum. Furosemide has been added as internal standard (IS) to improve precision. Fo...Continue Reading

References

Dec 1, 1994·The Analyst·E MärtlbauerR Dietrich
Dec 5, 2000·Journal of Chromatography. B, Biomedical Sciences and Applications·D A Stead
Apr 9, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Abdulsalam I Al-AmoudHenry Chrystyn
Oct 29, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Chia-Hsien FengSu-Hwei Chen
Dec 7, 2002·Journal of Pharmaceutical and Biomedical Analysis·Ibrahim A Darwish
Dec 31, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Y H LongJ Hoogmartens

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Citations

Nov 6, 2010·Biosensors & Bioelectronics·Eva González-FernándezPaulino Tuñón-Blanco
Mar 18, 2008·Journal of Chromatography. a·Yi-Fang LinSarah Y Chang
Jun 1, 2005·Journal of Pharmaceutical and Biomedical Analysis·Nan SunBao-Xiang Hu
Feb 24, 2015·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Hytham M Ahmed, Wael B Ebeid
Dec 3, 2014·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Lingyun ChenMei Shen
Jan 8, 2008·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Milly E Attema-de JongeEric J F Franssen

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