Bioanalysis of underivatized amino acids in non-invasive exhaled breath condensate samples using liquid chromatography coupled with tandem mass spectrometry

Journal of Chromatography. a
Lucyna KoniecznaTomasz Bączek

Abstract

Exhaled breath condensate (EBC) is receiving increased attention as a novel, entirely non-invasive technique for collecting biomarker samples. This increased attention is due to the fact that EBC is simple, effort independent, rapid, can be repeated frequently, and can be performed on young children and patients suffering from a variety of diseases. By having a subject breathe tidally through a cooling system for 15-20 min, a sufficient amount of condensate is collected for analysis of biomarkers in clinical studies. However, bioanalysis of EBC involves an unavoidable sample preparation step due to the low concentration of its components. Thus, there is a need for a new and more sensitive analytical method of assessing EBC samples. While researchers have considered analyses of single and small quantities of amino acids - for example, those connected with leukemia - no one has previously attempted to simultaneously analyze a panel of 23 amino acids. Moreover, the present study is well-justified, as prior studies focusing on single amino acids and leukemia at the moment of diagnosis and during chemotherapy (33 days of treatment) are inconsistent. In the present study, amino acids were separated using an XBridge Amide column (3 mm...Continue Reading

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Citations

Jul 27, 2018·Analytical and Bioanalytical Chemistry·Elaheh RahimpourAbolghasem Jouyban
Jul 2, 2019·Journal of Pharmaceutical and Biomedical Analysis·Yuanyuan FuZhiying Mao
Nov 5, 2019·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Juan LiHong-Min Liu
Mar 12, 2021·Critical Reviews in Analytical Chemistry·Maryam KhoubnasabjafariAbolghasem Jouyban
Nov 9, 2018·Analytical Chemistry·Valentina D'AtriDavy Guillarme

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