Bioanalytical approaches for the detection of protein acetylation-related enzymes

Analytical and Bioanalytical Chemistry
Pei LiShouzhuo Yao

Abstract

Reversible protein acetylation catalyzed by histone acetyltransferases (HATs) and histone deacetylases (HDACs) is an essential post-translational modification (PTM) mechanism which correlates largely with epigenetic gene regulation such as transcriptional activation, DNA replication, histone deposition, and DNA repair. Dysfunction of histone acetylation and the aberrant activity of HATs/HDACs is often associated with the pathogenesis of numerous diseases, especially cancer. Therefore, developing potent and specific analytical methods for HATs/HDACs is important for fundamental biochemical research, disease diagnosis and treatment, and drug development. This paper briefly summarizes the general design strategies used in HAT/HDAC sensors, gives a systematic overview of recent advances in the analytical methods for HAT/HDAC enzymatic analysis, classifies these methods by their biorecognition mechanisms and relative applications either in vitro or in living cells, then outlines challenges faced by these bioanalytical methods and offers perspectives on future developments.

References

May 3, 2000·Analytical Biochemistry·Y KimJ M Denu
Aug 11, 2001·Science·T Jenuwein, C D Allis
Feb 8, 2003·Chemistry & Biology·Dennis WegenerAndreas Schwienhorst
Jul 5, 2003·Analytical Biochemistry·Birgit HeltwegManfred Jung
Jul 12, 2003·Journal of Biomolecular Screening·Birgit Heltweg, Manfred Jung
Sep 27, 2003·Analytical Biochemistry·Dennis WegenerAndreas Schwienhorst
Sep 24, 2004·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Xiang-Jiao Yang
May 18, 2005·Angewandte Chemie·Markus BielAthanassios Giannis
Jan 26, 2007·Analytical Biochemistry·Daniel RiesterFranz-Josef Meyer-Almes
Aug 19, 2007·Journal of Biotechnology·David KuningerPeter Rotwein
Jun 19, 2008·Analytical Biochemistry·Jiang Wu, Yujun George Zheng
Jan 17, 2009·Bioconjugate Chemistry·Nan XieYujun George Zheng
May 22, 2009·Bioorganic & Medicinal Chemistry Letters·Daniel RiesterFranz-Josef Meyer-Almes
Jul 7, 2009·Drug Discovery Today·Frank J Dekker, Hidde J Haisma
Oct 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Kazuki SasakiMinoru Yoshida
Feb 20, 2010·Science·Shimin ZhaoKun-Liang Guan
Mar 16, 2012·Future Medicinal Chemistry·Berkley E GryderAdegboyega K Oyelere
Apr 14, 2012·Nature Reviews. Drug Discovery·Cheryl H ArrowsmithMatthieu Schapira
Jun 19, 2012·Journal of the American Chemical Society·Samuel A MinakerFraser Hof
Aug 21, 2012·Chemical Communications : Chem Comm·Alexander DoseDirk Schwarzer
Aug 25, 2012·Journal of the American Chemical Society·Reisuke BabaKazuya Kikuchi
Sep 11, 2012·Chembiochem : a European Journal of Chemical Biology·Beverley M DancyPhilip A Cole
Oct 25, 2012·Chemical Communications : Chem Comm·Koushik DharaKazuya Kikuchi
Nov 10, 2012·Analytical and Bioanalytical Chemistry·Tielong GaoYujun George Zheng
Aug 15, 2013·Scientific Reports·Yuko SatoHiroshi Kimura
Aug 19, 2014·Nature Reviews. Drug Discovery·Katrina J Falkenberg, Ricky W Johnstone
Sep 10, 2014·Nature Biotechnology·Mark Ratner

❮ Previous
Next ❯

Citations

Dec 12, 2020·Biosensors & Bioelectronics·Yan ChenHuanshun Yin

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.