Bioanalytical method development and validation of moxidectin in plasma by LC-MS/MS: Application to in vitro metabolism

Biomedical Chromatography : BMC
Y S ChhonkerDaryl J Murry

Abstract

Moxidectin (MOX) has recently been approved by the US Food and Drug Administration for the treatment of river blindness in select populations. It is also being evaluated as an alternative for the use of ivermectin, widespread resistance to which is becoming a global health issue. Moreover, MOX is becoming increasingly used as a prophylactic antiparasitic in the cattle industry. In this study, we developed and validated an LC-MS/MS method of MOX in human, monkey and mouse plasma. The separation was achieved on an ACE C18 (50 × 3.0 mm, 3 μm) column with isocratic elution using 0.1% acetic acid and methanol-acetonitrile (1:1, v/v) as mobile phase. MOX was quantitated using MS/MS with an electrospray ionization source operating in negative multiple reaction monitoring mode. The multiple reaction monitoring precursor ion → product ion transitions for MOX and abamectin (IS) were m/z 638.40 → 236.30 and m/z 871.50 → 565.35 respectively. The MS/MS response was linear over the concentration range 0.1-1000 ng/mL in plasma with a correlation coefficient (r2 ) of 0.997 or better. The within- and between-day precision (relative standard deviation, RSD) and accuracy were within the acceptable limits per US Food and Drug Administration guidel...Continue Reading

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References

Aug 1, 1986·Acta Pharmacologica Et Toxicologica·C BäärnhielmI Skånberg
Jan 1, 1994·Australian Veterinary Journal·P J Kieran
Jul 1, 1993·Pharmaceutical Research·B Davies, T Morris
Jun 2, 2001·Parasitology·K R BrownE A Ottesen
Oct 1, 2003·Journal of Clinical Pharmacology·Monette M CotreauUllrich S Schwertschlag
Oct 29, 2003·Trends in Parasitology·David H MolyneuxMark J Taylor
Mar 28, 2007·Analytica Chimica Acta·Bjorn J A BerendsenHans J A van Rhijn
Nov 7, 2008·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·R SmithH H Griffiths
Sep 18, 2010·Current Opinion in Infectious Diseases·Moses J Bockarie, Rinki M Deb
Jul 12, 2011·Analytica Chimica Acta·L GiannettiB Neri
Sep 28, 2013·Journal of Chromatographic Science·Qianqian XuFeng Li
Dec 1, 2012·International Journal for Parasitology. Drugs and Drug Resistance·Roger PrichardAnne Lespine
Apr 15, 2015·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·Nagsen GautamYazen Alnouti
Mar 18, 2016·PLoS Neglected Tropical Diseases·Kate E MounseyJames S McCarthy
Mar 14, 2017·Trends in Parasitology·Roz LaingEileen Devaney
Dec 12, 2017·European Journal of Medicinal Chemistry·Maddeboina KrishnaiahMartin Conda-Sheridan

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